Detailed information of TA system TA118836

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2389536..2390368
• Replicon: chromosome
• Accession: NZ_CP035855
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TJC5
• Locus tag: EXE31_RS11635
• Protein ID: WP_001094452.1
• Coordinates: 2389994..2390368 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I8Z144
• Locus tag: EXE31_RS11630
• Protein ID: WP_016255729.1
• Coordinates: 2389536..2389904 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS11605 (2386958)	2386958..2387191	+	234	WP_001117567.1	DUF905 family protein	-
EXE31_RS11610 (2387291)	2387291..2388109	+	819	WP_001234594.1	DUF932 domain-containing protein	-
EXE31_RS11615 (2388201)	2388201..2388686	+	486	WP_000213708.1	antirestriction protein	-
EXE31_RS11620 (2388701)	2388701..2389177	+	477	WP_001484796.1	RadC family protein	-
EXE31_RS11625 (2389240)	2389240..2389461	+	222	WP_000692315.1	DUF987 domain-containing protein	-
EXE31_RS11630 (2389536)	2389536..2389904	+	369	WP_016255729.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXE31_RS11635 (2389994)	2389994..2390368	+	375	WP_001094452.1	TA system toxin CbtA family protein	Toxin
EXE31_RS11640 (2390365)	2390365..2390856	+	492	WP_000977086.1	DUF5983 family protein	-
EXE31_RS11645 (2390867)	2390867..2391064	+	198	WP_000839264.1	DUF957 domain-containing protein	-
EXE31_RS11650 (2391149)	2391149..2391994	+	846	WP_001280506.1	DUF4942 domain-containing protein	-
EXE31_RS11660 (2392728)	2392728..2394979	+	2252	Protein_2288	alpha-amylase family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14014.86 Da
• Isoelectric Point: 7.2920

>T118836 WP_001094452.1 NZ_CP035855:2389994-2390368 [Escherichia coli TW11681]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T118836 NZ_CP035855:2389994-2390368 [Escherichia coli TW11681]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13750.53 Da
• Isoelectric Point: 6.7395

>AT118836 WP_016255729.1 NZ_CP035855:2389536-2389904 [Escherichia coli TW11681]
VSDTLHETTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118836 NZ_CP035855:2389536-2389904 [Escherichia coli TW11681]
GTGTCAGACACACTCCATGAGACAACTCATCCCGACGACAATCGCGGCCGCCCCTGGTGGGGACTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGA
GAACTGAATCCCCGTCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7Z7YIM6

Antitoxin

• AlphaFold DB: A0A7I8Z144