Detailed information of TA system TA118835

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1680002..1680837
• Replicon: chromosome
• Accession: NZ_CP035855
• Organism: Escherichia coli TW11681

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: EXE31_RS08340
• Protein ID: WP_000854752.1
• Coordinates: 1680460..1680837 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: EXE31_RS08335
• Protein ID: WP_001278232.1
• Coordinates: 1680002..1680370 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXE31_RS08305 (1676838)	1676838..1677293	+	456	WP_000581504.1	IrmA family protein	-
EXE31_RS08310 (1677372)	1677372..1677605	+	234	WP_001119729.1	DUF905 family protein	-
EXE31_RS08315 (1677705)	1677705..1678523	+	819	WP_001234620.1	DUF932 domain-containing protein	-
EXE31_RS08320 (1678578)	1678578..1679063	+	486	WP_000849582.1	antirestriction protein	-
EXE31_RS08325 (1679079)	1679079..1679555	+	477	WP_001186774.1	RadC family protein	-
EXE31_RS08330 (1679618)	1679618..1679839	+	222	WP_000692318.1	DUF987 domain-containing protein	-
EXE31_RS08335 (1680002)	1680002..1680370	+	369	WP_001278232.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
EXE31_RS08340 (1680460)	1680460..1680837	+	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
EXE31_RS08345 (1680834)	1680834..1680983	+	150	Protein_1636	DUF5983 family protein	-
EXE31_RS08350 (1681083)	1681083..1681163	+	81	Protein_1637	hypothetical protein	-
EXE31_RS08355 (1681961)	1681961..1682350	+	390	WP_001445118.1	IS110 family transposase	-
EXE31_RS08360 (1682509)	1682509..1682988	-	480	Protein_1639	IS3 family transposase	-
EXE31_RS08365 (1683072)	1683072..1684580	-	1509	WP_001189123.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T118835 WP_000854752.1 NZ_CP035855:1680460-1680837 [Escherichia coli TW11681]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T118835 NZ_CP035855:1680460-1680837 [Escherichia coli TW11681]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT118835 WP_001278232.1 NZ_CP035855:1680002-1680370 [Escherichia coli TW11681]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118835 NZ_CP035855:1680002-1680370 [Escherichia coli TW11681]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72