Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4639805..4640331 Replicon chromosome
Accession NZ_CP035826
Organism Escherichia coli strain 67-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EXA05_RS22595 Protein ID WP_000323025.1
Coordinates 4639805..4640092 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EXA05_RS22600 Protein ID WP_000534858.1
Coordinates 4640092..4640331 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA05_RS22555 (4634991) 4634991..4635203 + 213 WP_000087756.1 cold shock-like protein CspF -
EXA05_RS22560 (4635258) 4635258..4635347 + 90 WP_120795389.1 hypothetical protein -
EXA05_RS22565 (4635625) 4635625..4636377 - 753 WP_001047135.1 antitermination protein -
EXA05_RS22570 (4636391) 4636391..4637440 - 1050 WP_097343536.1 DUF968 domain-containing protein -
EXA05_RS22575 (4637442) 4637442..4637702 - 261 WP_097343537.1 hypothetical protein -
EXA05_RS22580 (4637722) 4637722..4638930 - 1209 WP_000343717.1 IS256-like element IS1414 family transposase -
EXA05_RS22585 (4639110) 4639110..4639361 - 252 WP_000980994.1 protein Rem -
EXA05_RS22590 (4639578) 4639578..4639733 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EXA05_RS22595 (4639805) 4639805..4640092 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EXA05_RS22600 (4640092) 4640092..4640331 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EXA05_RS22605 (4640356) 4640356..4640565 + 210 Protein_4429 protein YdfV -
EXA05_RS22610 (4640564) 4640564..4640692 - 129 Protein_4430 dicB transcriptional regulator DicC -
EXA05_RS22615 (4640776) 4640776..4641183 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
EXA05_RS22620 (4641350) 4641350..4641505 + 156 WP_000379575.1 DUF1391 family protein -
EXA05_RS26980 (4641507) 4641507..4641608 + 102 Protein_4433 protein YdfB -
EXA05_RS22630 (4642569) 4642569..4642757 + 189 WP_000854559.1 cell division inhibition protein DicB -
EXA05_RS22635 (4642754) 4642754..4642945 + 192 WP_001083278.1 lysis protein YdfD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4617407..4649617 32210
- flank IS/Tn - - 4637722..4638930 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T118659 WP_000323025.1 NZ_CP035826:c4640092-4639805 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T118659 NZ_CP035826:c4640092-4639805 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT118659 WP_000534858.1 NZ_CP035826:c4640331-4640092 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT118659 NZ_CP035826:c4640331-4640092 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References