Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4505965..4506506 Replicon chromosome
Accession NZ_CP035822
Organism Escherichia coli strain WS4437A-1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag EXA06_RS21775 Protein ID WP_000615983.1
Coordinates 4505965..4506243 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag EXA06_RS21780 Protein ID WP_000729703.1
Coordinates 4506246..4506506 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA06_RS21760 (4503547) 4503547..4504125 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EXA06_RS21765 (4504331) 4504331..4505098 + 768 WP_000333380.1 class II glutamine amidotransferase -
EXA06_RS21770 (4505069) 4505069..4505809 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EXA06_RS21775 (4505965) 4505965..4506243 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EXA06_RS21780 (4506246) 4506246..4506506 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EXA06_RS21785 (4506716) 4506716..4507465 + 750 WP_024168917.1 C40 family peptidase -
EXA06_RS21790 (4507641) 4507641..4508138 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EXA06_RS21795 (4508362) 4508362..4510455 - 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T118642 WP_000615983.1 NZ_CP035822:c4506243-4505965 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118642 NZ_CP035822:c4506243-4505965 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT118642 WP_000729703.1 NZ_CP035822:c4506506-4506246 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118642 NZ_CP035822:c4506506-4506246 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References