Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1586749..1587251 Replicon chromosome
Accession NZ_CP035822
Organism Escherichia coli strain WS4437A-1

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag EXA06_RS07945 Protein ID WP_000767835.1
Coordinates 1586749..1587003 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EXA06_RS07950 Protein ID WP_001259255.1
Coordinates 1587000..1587251 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA06_RS07925 (1583242) 1583242..1584600 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EXA06_RS07930 (1584590) 1584590..1584652 - 63 WP_010723108.1 membrane protein YoeI -
EXA06_RS07935 (1584867) 1584867..1585796 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
EXA06_RS07940 (1585842) 1585842..1586666 - 825 WP_000754737.1 SDR family oxidoreductase -
EXA06_RS07945 (1586749) 1586749..1587003 - 255 WP_000767835.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EXA06_RS07950 (1587000) 1587000..1587251 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EXA06_RS07955 (1587535) 1587535..1587585 + 51 WP_001364200.1 his operon leader peptide -
EXA06_RS07960 (1587731) 1587731..1588630 + 900 WP_000131774.1 ATP phosphoribosyltransferase -
EXA06_RS07965 (1588636) 1588636..1589940 + 1305 WP_001413482.1 histidinol dehydrogenase -
EXA06_RS07970 (1589937) 1589937..1591007 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
EXA06_RS07975 (1591007) 1591007..1592074 + 1068 WP_000080072.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.51 Da        Isoelectric Point: 7.2751

>T118634 WP_000767835.1 NZ_CP035822:c1587003-1586749 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118634 NZ_CP035822:c1587003-1586749 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118634 WP_001259255.1 NZ_CP035822:c1587251-1587000 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118634 NZ_CP035822:c1587251-1587000 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References