Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 159357..160000 Replicon plasmid pE7476_162
Accession NZ_CP035818
Organism Escherichia coli strain E7476

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag EXA08_RS25345 Protein ID WP_001356337.1
Coordinates 159584..160000 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag EXA08_RS25340 Protein ID WP_024220872.1
Coordinates 159357..159587 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA08_RS25310 (154485) 154485..154760 + 276 WP_000239529.1 hypothetical protein -
EXA08_RS25315 (154898) 154898..155203 - 306 Protein_180 site-specific integrase -
EXA08_RS25320 (155294) 155294..156522 + 1229 WP_089541817.1 IS3 family transposase -
EXA08_RS25325 (156534) 156534..157016 - 483 Protein_182 site-specific integrase -
EXA08_RS25330 (157018) 157018..157431 - 414 WP_000465050.1 hypothetical protein -
EXA08_RS25335 (158057) 158057..159076 + 1020 Protein_184 IS110 family transposase -
EXA08_RS25340 (159357) 159357..159587 + 231 WP_024220872.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EXA08_RS25345 (159584) 159584..160000 + 417 WP_001356337.1 type II toxin-antitoxin system VapC family toxin Toxin
EXA08_RS25350 (159967) 159967..160314 - 348 Protein_187 helix-turn-helix domain-containing protein -
EXA08_RS25635 (160426) 160426..160560 + 135 Protein_188 hypothetical protein -
EXA08_RS25360 (161868) 161868..162143 + 276 Protein_190 integrase core domain-containing protein -
EXA08_RS25365 (162152) 162152..162301 - 150 Protein_191 transposase -
EXA08_RS25640 (162617) 162617..162826 - 210 WP_000129488.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - etpB / etpA / cfaA / csaC / csaE 1..162860 162860
- inside IScluster/Tn - - 149678..162826 13148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15059.51 Da        Isoelectric Point: 7.7814

>T118596 WP_001356337.1 NZ_CP035818:159584-160000 [Escherichia coli]
MKKTYMLDTCICSFIMREQPEAVLNRLEQTVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLIDAFCARLDAVLPWDRT
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTSNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T118596 NZ_CP035818:159584-160000 [Escherichia coli]
ATGAAGAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCAGAAGCTGTGCTGAATCGCCT
GGAGCAGACGGTGCTGCGCGGTCACCGTATCGTGGTCTCAGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCACCGCGTCACGTACAGTTGATTGACGCGTTCTGCGCCCGGCTCGATGCCGTCCTGCCCTGGGACCGCACC
GCGGTGGATGCCACCACAGAGATTAAGGTGGCCCTGCGCCTGGCCGGCACGCCGATCGGTCCGAACGACACCGCCATTGC
CGGACACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAGTAATGTGAGAGAGTTTGCACGGGTGCCAGGCCTGGTGCTGG
AAGACTGGGTTAACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8792.91 Da        Isoelectric Point: 4.4992

>AT118596 WP_024220872.1 NZ_CP035818:159357-159587 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPSWGSFAQLEKADPDFMAERDDVVTDEGRFEL

Download         Length: 231 bp

>AT118596 NZ_CP035818:159357-159587 [Escherichia coli]
ATGAGAACAGTATCCATTTTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGGCCGAGCTGGGGCTCGTTCGCGCAGCTTG
AAAAGGCCGATCCAGACTTTATGGCAGAGCGCGATGACGTTGTCACCGACGAAGGACGCTTTGAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References