Detailed information of TA system TA118548

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1204809..1205600
• Replicon: chromosome
• Accession: NZ_CP035812
• Organism: Escherichia coli strain B7A

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EXA09_RS05580
• Protein ID: WP_001296916.1
• Coordinates: 1204809..1205234 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EXA09_RS05585
• Protein ID: WP_001296928.1
• Coordinates: 1205265..1205600 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA09_RS25970 (1201170)	1201170..1201331	-	162	Protein_1074	virulence RhuM family protein	-
EXA09_RS05560 (1201654)	1201654..1202040	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
EXA09_RS05565 (1202663)	1202663..1203394	-	732	WP_001216640.1	retron system putative HNH endonuclease	-
EXA09_RS05570 (1203391)	1203391..1203612	-	222	WP_126125326.1	hypothetical protein	-
EXA09_RS05575 (1203597)	1203597..1204788	-	1192	Protein_1078	AAA family ATPase	-
EXA09_RS05580 (1204809)	1204809..1205234	-	426	WP_001296916.1	TA system toxin CbtA family protein	Toxin
EXA09_RS05585 (1205265)	1205265..1205600	-	336	WP_001296928.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EXA09_RS05590 (1205600)	1205600..1206073	-	474	WP_001296921.1	DNA repair protein RadC	-
EXA09_RS05595 (1206103)	1206103..1206921	-	819	WP_001296926.1	DUF932 domain-containing protein	-
EXA09_RS05600 (1207158)	1207158..1208111	-	954	WP_000290409.1	hypothetical protein	-
EXA09_RS05605 (1208704)	1208704..1209318	+	615	WP_000772911.1	inovirus Gp2 family protein	-
EXA09_RS05610 (1209436)	1209436..1209654	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16565.22 Da
• Isoelectric Point: 10.3624

>T118548 WP_001296916.1 NZ_CP035812:c1205234-1204809 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQERIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDVLRARQATGLRIQCKNTNRISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T118548 NZ_CP035812:c1205234-1204809 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACGCATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAGTTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATGTTTTACGGGCGCGACAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATAGAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12565.57 Da
• Isoelectric Point: 7.7245

>AT118548 WP_001296928.1 NZ_CP035812:c1205600-1205265 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKHLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT118548 NZ_CP035812:c1205600-1205265 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACATCTGGAGCTGAAACTGGCTGACGGTGAACTCTCTCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA