Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1353978..1354773 Replicon chromosome
Accession NZ_CP035767
Organism Escherichia coli O145 strain RM12522-C8

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag EW647_RS06965 Protein ID WP_000854914.1
Coordinates 1354399..1354773 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag EW647_RS06960 Protein ID WP_001280953.1
Coordinates 1353978..1354352 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW647_RS06930 1349333..1350238 + 906 WP_000203541.1 chemotaxis protein -
EW647_RS06935 1350235..1351305 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
EW647_RS06940 1351645..1352463 + 819 WP_001234682.1 DUF945 domain-containing protein -
EW647_RS06945 1352554..1353039 + 486 WP_000214398.1 antirestriction protein -
EW647_RS06950 1353055..1353531 + 477 WP_001186738.1 RadC family protein -
EW647_RS06955 1353594..1353815 + 222 WP_000692323.1 DUF987 domain-containing protein -
EW647_RS06960 1353978..1354352 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EW647_RS06965 1354399..1354773 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
EW647_RS06970 1354770..1355261 + 492 WP_000976857.1 hypothetical protein -
EW647_RS06975 1355273..1355470 + 198 WP_000839282.1 DUF957 domain-containing protein -
EW647_RS06980 1355555..1356397 + 843 WP_001280481.1 DUF4942 domain-containing protein -
EW647_RS06990 1356868..1357806 + 939 WP_000351301.1 glyoxylate/hydroxypyruvate reductase GhrA -
EW647_RS06995 1357861..1358598 + 738 WP_000283664.1 zinc-binding phosphatase -
EW647_RS07000 1358622..1359176 + 555 WP_001001917.1 molecular chaperone YcdY -
EW647_RS07005 1359278..1359769 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1353055..1364706 11651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T118407 WP_000854914.1 NZ_CP035767:1354399-1354773 [Escherichia coli O145]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T118407 NZ_CP035767:1354399-1354773 [Escherichia coli O145]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT118407 WP_001280953.1 NZ_CP035767:1353978-1354352 [Escherichia coli O145]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT118407 NZ_CP035767:1353978-1354352 [Escherichia coli O145]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References