Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 541468..542234 Replicon chromosome
Accession NZ_CP035689
Organism Vibrio metoecus strain 08-2459

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag EWA65_RS15955 Protein ID WP_000982260.1
Coordinates 541737..542234 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0Q0PQR2
Locus tag EWA65_RS15950 Protein ID WP_000246255.1
Coordinates 541468..541740 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWA65_RS15910 536623..536883 - 261 WP_195708054.1 DUF3709 domain-containing protein -
EWA65_RS15915 536956..537066 - 111 WP_141229692.1 DUF3265 domain-containing protein -
EWA65_RS15920 537063..538070 - 1008 WP_154139256.1 hypothetical protein -
EWA65_RS15925 538240..538785 - 546 WP_099609953.1 hypothetical protein -
EWA65_RS15930 538932..539318 - 387 WP_195708055.1 VOC family protein -
EWA65_RS15935 539475..540167 - 693 WP_148494390.1 hypothetical protein -
EWA65_RS15940 540364..540534 - 171 Protein_536 DUF645 family protein -
EWA65_RS15945 540898..541078 - 181 Protein_537 DUF645 family protein -
EWA65_RS15950 541468..541740 + 273 WP_000246255.1 DUF1778 domain-containing protein Antitoxin
EWA65_RS15955 541737..542234 + 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
EWA65_RS15960 542267..542359 - 93 WP_195708056.1 DUF3265 domain-containing protein -
EWA65_RS15965 542383..542799 - 417 WP_055028356.1 hypothetical protein -
EWA65_RS15970 542789..543280 - 492 WP_055028355.1 SocA family protein -
EWA65_RS15975 543550..543942 - 393 WP_041202691.1 nuclear transport factor 2 family protein -
EWA65_RS15980 544113..545335 - 1223 Protein_544 DUF3883 domain-containing protein -
EWA65_RS15985 545526..545897 - 372 WP_055028354.1 hypothetical protein -
EWA65_RS15990 545942..546053 - 112 Protein_546 DUF3265 domain-containing protein -
EWA65_RS15995 546068..546436 - 369 WP_154139258.1 VOC family protein -
EWA65_RS16005 546862..547020 - 159 WP_154139259.1 DUF3265 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 453640..607302 153662
- inside Integrative and Conjugative Element - hlyA 504927..845208 340281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T118263 WP_000982260.1 NZ_CP035689:541737-542234 [Vibrio metoecus]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T118263 NZ_CP035689:541737-542234 [Vibrio metoecus]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTATACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCTGTTGTCATTGTTGATGCTAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9713.24 Da        Isoelectric Point: 6.2988

>AT118263 WP_000246255.1 NZ_CP035689:541468-541740 [Vibrio metoecus]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT118263 NZ_CP035689:541468-541740 [Vibrio metoecus]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTACTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAACTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q0PQR2

References