Detailed information of TA system TA118196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2111357..2113077
• Replicon: chromosome
• Accession: NZ_CP035668
• Organism: Edwardsiella piscicida strain MS-18-199

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A411H3H5
• Locus tag: EVK84_RS09655
• Protein ID: WP_015683486.1
• Coordinates: 2111357..2112013 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: EVK84_RS09660
• Protein ID: WP_041692662.1
• Coordinates: 2112010..2113077 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVK84_RS09635	2106559..2106915	+	357	Protein_1828	integrase	-
EVK84_RS09640	2107267..2107506	+	240	WP_012849883.1	hypothetical protein	-
EVK84_RS09645	2107545..2109983	+	2439	WP_015683488.1	type I restriction-modification system subunit M	-
EVK84_RS09650	2110034..2111344	+	1311	WP_071819616.1	restriction endonuclease subunit S	-
EVK84_RS09655	2111357..2112013	+	657	WP_015683486.1	DUF4433 domain-containing protein	Toxin
EVK84_RS09660	2112010..2113077	+	1068	WP_041692662.1	macro domain-containing protein	Antitoxin
EVK84_RS09665	2113241..2116336	+	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
EVK84_RS09670	2116336..2117052	+	717	WP_012849890.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2091951..2132210	40259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25046.43 Da
• Isoelectric Point: 6.9045

>T118196 WP_015683486.1 NZ_CP035668:2111357-2112013 [Edwardsiella piscicida]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSQVQAVNWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSSLRSVAAQNAPFVFTDSHAYYNWANYYTRLEDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T118196 NZ_CP035668:2111357-2112013 [Edwardsiella piscicida]
ATGGCTTATGACTACAGCGCCAGTCTCAACCCGCAAAAAGCCCTGATCTGGCGCATTGTCCATCGGGATAATATCCCGTG
GATACTGGACAACGGCTTGCACTGCGGCAACAGTCAAGTGCAGGCGGTAAACTGGATCAATATCGGCAACCCGGAACTTA
TCGGCAAACGCGCCGGGCATCCGGTGCCTGTCGGGACGGGCGGGACGCTGCATGACTACGTGCCGTTTTACTTTACCCCC
TTTTCGCCGATGCTGATGAATATCCACAGCGGGCGTGGCGGCATTACGCAGCGCCCCAACGAGGAAATCGTCATTCTGGT
CAGCAGCCTGCGAAGCGTGGCGGCGCAAAACGCCCCCTTCGTGTTTACCGACAGCCATGCCTACTATAACTGGGCCAACT
ACTACACCCGCCTGGAGGACCTGAACCAGATCGACTGGTCTATCTTACAGGCGCGGGATTTCCGTCGCGATCCGGACGAT
CCGGCGAAGTTTGAACGCTATCAGGCCGAGGCGTTAATCTGGCAGCACTGCCCCATCACACTGCTGGTCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGCTGTCGCAACGTAATCTGACAATGCCCGTTCACGCACGGGCCG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40668.02 Da
• Isoelectric Point: 6.9067

>AT118196 WP_041692662.1 NZ_CP035668:2112010-2113077 [Edwardsiella piscicida]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIELHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT118196 NZ_CP035668:2112010-2113077 [Edwardsiella piscicida]
ATGATCACATATACACAAGGCAATTTACTGGACGCGCCCGTGGAAGCACTGGTGAATACGGTGAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTTAAAGAGCGCTTCCCCGACAATATGAAAGCCTATGCCCACGCCTGCAAACAAAAGC
AGGTGATGACCGGCAAGATGTTTATTACCGAAACCGGCGAGTTGATGGGGCCGCGCTGGATCGTCAACTTCCCTACCAAG
CAGCACTGGCGCGCCGACTCTCGCATGGCGTGGATCGAGGACGGCTTGCAGGACCTGCGCCGCTTTTTGATCGAAAAGAA
AGTGCGGTCCATCGCCATTCCCCCGCTGGGCGCGGGCAACGGCGGCCTGAACTGGCCGGACGTCCGGGCGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATCCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCAGC
GGTGTAAAAAAGCTCACTCCCGCCAGAGCGATGATCGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCGTGGCTGCTACAACGCGCCATTGAACTGCACCAGCAGGAGGACGTGCTAAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTATGCTCCTAACCTGAACCATCTGCTTAACGCGCTGGACGGTACCTATCTGAAA
GCCGAAAAGCGTATCCCGGACAGCCAGCCACTGGATGTGATCTGGTTTAACGATCGGGAAAAAGAACACATCAACCTATG
GCTGAACAGCGAAGCCAAAGCGTGGTTGCCAGCGCTTGAGCAGGTCAGCCAGTTGATTGACGGTTTTGAATCGCCGTTTG
GTCTGGAGCTGTTGGCGACCGTTGACTGGTTGTTGACCCGGGGACAATGCCAGCCCACGCTGGACTCCGTGAAAGAGGGG
TTGCATCAGTGGCCTGCGGGAGAACCTTGGGCCAGCCGTAAGATAAAGCTGTTTGACGATAAGAATCTGCAATTCGCCAT
TAACCGCGTGATGGAGTTTCATTGCTGA

Structures

Toxin

• AlphaFold DB: A0A411H3H5