Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49727..50252 Replicon plasmid pAP855
Accession NZ_CP035384
Organism Klebsiella pneumoniae strain AP8555

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag ES964_RS27130 Protein ID WP_025714517.1
Coordinates 49947..50252 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag ES964_RS27125 Protein ID WP_001568025.1
Coordinates 49727..49945 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES964_RS27105 47409..48437 - 1029 WP_001568023.1 Abi family protein -
ES964_RS27110 48578..49147 - 570 WP_016162066.1 hypothetical protein -
ES964_RS27125 49727..49945 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ES964_RS27130 49947..50252 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
ES964_RS27135 50421..50816 + 396 WP_017899885.1 hypothetical protein -
ES964_RS27140 50843..51157 + 315 WP_053389906.1 hypothetical protein -
ES964_RS27145 51168..52184 + 1017 WP_017899884.1 hypothetical protein -
ES964_RS27150 52382..53176 + 795 WP_004197635.1 site-specific integrase -
ES964_RS30040 53672..53974 - 303 WP_071571079.1 hypothetical protein -
ES964_RS27160 53971..54597 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..357837 357837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T117710 WP_025714517.1 NZ_CP035384:49947-50252 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T117710 NZ_CP035384:49947-50252 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTTTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT117710 WP_001568025.1 NZ_CP035384:49727-49945 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT117710 NZ_CP035384:49727-49945 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References