Detailed information of TA system TA117605

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69429..69682
• Replicon: plasmid pD72-F33
• Accession: NZ_CP035314
• Organism: Escherichia coli strain D72

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: ERL60_RS23295
• Protein ID: WP_001312851.1
• Coordinates: 69533..69682 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69429..69488 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERL60_RS23265	66156..66902	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
ERL60_RS23270	66957..67517	+	561	WP_000139328.1	fertility inhibition protein FinO	-
ERL60_RS23275	67649..67849	+	201	WP_015059022.1	hypothetical protein	-
ERL60_RS23280	68235..68834	+	600	WP_032083981.1	hypothetical protein	-
-	69429..69488	-	60	NuclAT_1	-	Antitoxin
-	69429..69488	-	60	NuclAT_1	-	Antitoxin
-	69429..69488	-	60	NuclAT_1	-	Antitoxin
-	69429..69488	-	60	NuclAT_1	-	Antitoxin
ERL60_RS23295	69533..69682	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
ERL60_RS23300	69966..70214	+	249	WP_000083837.1	replication regulatory protein RepA	-
ERL60_RS23310	70459..70533	+	75	WP_001375168.1	RepA leader peptide Tap	-
ERL60_RS23315	70526..71383	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
ERL60_RS23325	72322..72975	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
ERL60_RS23330	73068..73325	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
ERL60_RS23335	73258..73659	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
ERL60_RS23340	73908..74323	+	416	Protein_91	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-55	-	1..89963	89963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T117605 WP_001312851.1 NZ_CP035314:69533-69682 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T117605 NZ_CP035314:69533-69682 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT117605 NZ_CP035314:c69488-69429 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin