Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1300803..1301377 Replicon chromosome
Accession NZ_CP035284
Organism Paracoccus versutus strain UW1

Toxin (Protein)


Gene name mazF Uniprot ID A0A3E0BZ21
Locus tag EQ718_RS06580 Protein ID WP_036750899.1
Coordinates 1301045..1301377 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3E0BWP9
Locus tag EQ718_RS06575 Protein ID WP_036750897.1
Coordinates 1300803..1301045 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ718_RS06550 (EQ718_06505) 1296357..1296623 - 267 WP_036750880.1 conjugal transfer protein TraD -
EQ718_RS06555 (EQ718_06510) 1296625..1297056 - 432 WP_243700178.1 conjugal transfer protein TraD -
EQ718_RS06560 (EQ718_06515) 1297133..1300141 + 3009 WP_036750893.1 Ti-type conjugative transfer relaxase TraA -
EQ718_RS06565 (EQ718_06520) 1300272..1300487 + 216 WP_036750895.1 AlpA family transcriptional regulator -
EQ718_RS06570 (EQ718_06525) 1300474..1300692 + 219 WP_157034055.1 hypothetical protein -
EQ718_RS06575 (EQ718_06530) 1300803..1301045 + 243 WP_036750897.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EQ718_RS06580 (EQ718_06535) 1301045..1301377 + 333 WP_036750899.1 endoribonuclease MazF Toxin
EQ718_RS06585 (EQ718_06540) 1301391..1303229 + 1839 WP_084196840.1 recombinase family protein -
EQ718_RS06590 (EQ718_06545) 1303159..1303656 - 498 WP_147307282.1 hypothetical protein -
EQ718_RS06595 (EQ718_06550) 1303830..1304498 - 669 WP_166436096.1 ANTAR domain-containing protein -
EQ718_RS06600 (EQ718_06555) 1304573..1305844 - 1272 WP_084196842.1 transporter substrate-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1288198..1321137 32939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-109)

Antitoxin

SpoVT-AbrB domain

(6-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12112.23 Da        Isoelectric Point: 10.5364

>T117541 WP_036750899.1 NZ_CP035284:1301045-1301377 [Paracoccus versutus]
MAGWVPDSGDIVWLNFDPQAGHEQAGHRPALVLSPARYNGLRGMMLCCPLTTKLKGYPFEVVISQNPPSAVLVDQVKSLD
WRARKARHKGKVTAVVLADVRAKLRALLQI

Download         Length: 333 bp

>T117541 NZ_CP035284:1301045-1301377 [Paracoccus versutus]
ATGGCGGGATGGGTGCCGGACAGCGGCGACATCGTGTGGCTGAATTTCGATCCGCAGGCCGGGCACGAGCAAGCTGGTCA
TCGTCCCGCTCTGGTGCTGTCGCCCGCCCGCTACAACGGGCTGCGGGGCATGATGCTTTGCTGCCCGTTGACCACGAAAC
TCAAGGGCTACCCGTTTGAAGTCGTCATCAGCCAGAACCCGCCAAGCGCGGTTCTGGTCGATCAGGTCAAAAGCCTCGAT
TGGCGTGCGCGCAAGGCCAGGCACAAGGGCAAGGTGACTGCCGTGGTGCTCGCCGACGTTCGTGCCAAGCTCAGGGCACT
GTTGCAAATCTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8791.89 Da        Isoelectric Point: 4.5349

>AT117541 WP_036750897.1 NZ_CP035284:1300803-1301045 [Paracoccus versutus]
MQVSIKKWGNSASVRIPHSVMTAANLHVDQAVEVREEDGRIVIEPVQGLSYDLDKLLSGMTPDTFHDEADFGAPAGKEVW

Download         Length: 243 bp

>AT117541 NZ_CP035284:1300803-1301045 [Paracoccus versutus]
ATGCAAGTGAGCATAAAGAAATGGGGCAACAGCGCCTCGGTGCGGATTCCGCATTCGGTGATGACTGCCGCCAACCTGCA
TGTCGATCAGGCGGTGGAGGTGCGTGAGGAAGATGGCCGGATAGTTATCGAACCTGTCCAGGGCCTTTCCTATGATCTCG
ACAAGCTGCTGTCCGGGATGACGCCCGACACCTTCCATGACGAGGCGGATTTCGGCGCTCCGGCTGGCAAGGAAGTCTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3E0BZ21

Antitoxin

Source ID Structure
AlphaFold DB A0A3E0BWP9

References