Detailed information of TA system TA117378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1327051..1328639
• Replicon: chromosome
• Accession: NZ_CP035207
• Organism: Klebsiella quasipneumoniae strain TH114

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EQH50_RS06405
• Protein ID: WP_087833293.1
• Coordinates: 1327051..1328373 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: EQH50_RS06410
• Protein ID: WP_004205921.1
• Coordinates: 1328373..1328639 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH50_RS06375	1323875..1324123	+	249	WP_004205912.1	hypothetical protein	-
EQH50_RS06380	1324414..1324632	+	219	WP_002904808.1	ParD-like family protein	-
EQH50_RS06385	1324613..1325395	+	783	WP_049003659.1	type I methionyl aminopeptidase	-
EQH50_RS06390	1325496..1325600	+	105	Protein_1243	helix-turn-helix domain-containing protein	-
EQH50_RS06400	1325742..1327034	+	1293	WP_099441800.1	glycoside hydrolase family 10 protein	-
EQH50_RS06405	1327051..1328373	-	1323	WP_087833293.1	type II toxin-antitoxin system HipA family toxin	Toxin
EQH50_RS06410	1328373..1328639	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
EQH50_RS06415	1328922..1329248	-	327	WP_069602708.1	hydroxyisourate hydrolase	-
EQH50_RS06420	1329245..1329745	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
EQH50_RS06425	1329723..1331102	-	1380	WP_023318211.1	purine permease	-
EQH50_RS06430	1331448..1332602	+	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
EQH50_RS06435	1332583..1333506	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49099.36 Da
• Isoelectric Point: 8.2655

>T117378 WP_087833293.1 NZ_CP035207:c1328373-1327051 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILDEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Nucleotide

• Length: 1323 bp

>T117378 NZ_CP035207:c1328373-1327051 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATTTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGTGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGTTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATAATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGACGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGAACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT117378 WP_004205921.1 NZ_CP035207:c1328639-1328373 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT117378 NZ_CP035207:c1328639-1328373 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9