Detailed information of TA system TA117112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4503406..4504208
• Replicon: chromosome
• Accession: NZ_CP034998
• Organism: Rhizobium acidisoli strain FH23

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A0N0LFY5
• Locus tag: CO657_RS21960
• Protein ID: WP_054182079.1
• Coordinates: 4503681..4504208 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: CO657_RS21955
• Protein ID: WP_054182080.1
• Coordinates: 4503406..4503684 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO657_RS21925	4498751..4499761	+	1011	WP_054182083.1	helix-turn-helix domain-containing protein	-
CO657_RS21930	4499918..4500772	+	855	WP_003589111.1	helix-turn-helix transcriptional regulator	-
CO657_RS21935	4500811..4501356	+	546	WP_003589109.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
CO657_RS21940	4501382..4502008	-	627	WP_054182082.1	dihydrofolate reductase family protein	-
CO657_RS21945	4502098..4502496	-	399	WP_054182081.1	VOC family protein	-
CO657_RS21950	4502594..4503295	+	702	WP_003589103.1	YafY family transcriptional regulator	-
CO657_RS21955	4503406..4503684	+	279	WP_054182080.1	DUF1778 domain-containing protein	Antitoxin
CO657_RS21960	4503681..4504208	+	528	WP_054182079.1	hypothetical protein	Toxin
CO657_RS21965	4504369..4505931	-	1563	WP_054182078.1	glutamine-hydrolyzing GMP synthase	-
CO657_RS21970	4506044..4506682	-	639	WP_003589098.1	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-
CO657_RS21975	4506679..4507128	-	450	WP_003544704.1	PaaI family thioesterase	-
CO657_RS21980	4507360..4508436	+	1077	WP_054182077.1	DUF2252 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18712.40 Da
• Isoelectric Point: 7.3519

>T117112 WP_054182079.1 NZ_CP034998:4503681-4504208 [Rhizobium acidisoli]
MTLPAWREEAIAKSHDRQSFDCGDLAMNDFFRRYARQSHEQNASKTFCAIDASTPNRVLGFYTVAPSSVAHEGVPASMTK
GLAQHEVPGFKLARIATDISVAGHGLGGQLLAAAALRCLRIASEAGGVLLIIDAKGERAAQWYVSYGAEPLENQPLTLVM
PLATFAADLKGKGLL

Nucleotide

• Length: 528 bp

>T117112 NZ_CP034998:4503681-4504208 [Rhizobium acidisoli]
ATGACGCTGCCCGCCTGGAGAGAGGAAGCCATCGCGAAATCGCATGATCGCCAGTCCTTCGACTGCGGCGATCTGGCGAT
GAACGACTTTTTCCGACGCTACGCGCGTCAAAGCCACGAACAGAACGCCTCAAAGACCTTCTGCGCGATCGACGCCTCTA
CGCCCAATCGTGTGCTGGGGTTCTACACTGTCGCCCCCTCCTCCGTGGCACATGAAGGTGTGCCGGCGTCGATGACGAAG
GGGCTGGCCCAGCACGAAGTTCCTGGGTTCAAGCTGGCTCGGATCGCAACCGATATCTCTGTAGCGGGCCATGGTTTGGG
TGGACAGCTCCTCGCGGCCGCGGCGCTTCGCTGCCTGCGGATCGCGTCCGAAGCAGGCGGCGTTCTGCTGATTATCGACG
CGAAGGGCGAGCGCGCGGCCCAATGGTATGTCAGCTATGGGGCCGAGCCGCTGGAGAACCAGCCGCTTACGCTTGTCATG
CCGCTCGCCACCTTCGCCGCTGATCTCAAGGGCAAGGGTCTTCTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10092.71 Da
• Isoelectric Point: 10.4718

>AT117112 WP_054182080.1 NZ_CP034998:4503406-4503684 [Rhizobium acidisoli]
MPQAAVDDNKRMNLRVSPETKAKLVRAAALRNTDLTNFVTQTALREAEAVIEAAEVVRLKARDHARVLELLENPPKPNAK
LRAAIAALPDTL

Nucleotide

• Length: 279 bp

>AT117112 NZ_CP034998:4503406-4503684 [Rhizobium acidisoli]
ATGCCCCAAGCTGCCGTAGATGACAACAAGCGTATGAATCTGCGGGTCTCCCCGGAAACGAAGGCCAAGCTCGTGCGCGC
CGCTGCGCTGCGCAACACCGACTTGACCAACTTCGTGACCCAGACCGCCTTGCGCGAAGCCGAAGCCGTGATCGAGGCGG
CCGAGGTCGTTCGCCTGAAGGCGCGCGATCATGCGCGCGTGCTGGAACTGCTGGAAAATCCGCCCAAGCCCAACGCGAAG
CTGCGGGCGGCGATCGCGGCGTTGCCCGATACTCTATGA

Structures

Toxin

• AlphaFold DB: A0A0N0LFY5