Detailed information of TA system TA117062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1344382..1345007
• Replicon: chromosome
• Accession: NZ_CP034958
• Organism: Escherichia coli strain SCEC020026

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7V14_RS07600
• Protein ID: WP_000911330.1
• Coordinates: 1344609..1345007 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C7V14_RS07595
• Protein ID: WP_000450524.1
• Coordinates: 1344382..1344609 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V14_RS07570	1340185..1340655	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C7V14_RS07575	1340655..1341227	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C7V14_RS07580	1341373..1342251	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C7V14_RS07585	1342268..1343302	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C7V14_RS07590	1343515..1344228	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C7V14_RS07595	1344382..1344609	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C7V14_RS07600	1344609..1345007	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C7V14_RS07605	1345154..1346017	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
C7V14_RS07610	1346032..1348047	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
C7V14_RS07615	1348121..1348462	+	342	Protein_1272	esterase	-
C7V14_RS07620	1348544..1349290	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T117062 WP_000911330.1 NZ_CP034958:1344609-1345007 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T117062 NZ_CP034958:1344609-1345007 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT117062 WP_000450524.1 NZ_CP034958:1344382-1344609 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT117062 NZ_CP034958:1344382-1344609 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33