Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 22227..22867 Replicon plasmid p1-AD3
Accession NZ_CP034903
Organism Roseomonas mucosa strain AD3

Toxin (Protein)


Gene name vapC Uniprot ID A0A379PMP7
Locus tag RMP42_RS19445 Protein ID WP_019463224.1
Coordinates 22227..22634 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A379PNA3
Locus tag RMP42_RS19450 Protein ID WP_019463225.1
Coordinates 22631..22867 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RMP42_RS19425 (RMP42_03202) 17629..20304 - 2676 WP_081798677.1 CRISPR-associated helicase/endonuclease Cas3 -
RMP42_RS19430 (RMP42_03203a) 20362..20772 - 411 WP_019463221.1 type II toxin-antitoxin system VapC family toxin -
RMP42_RS19435 (RMP42_03204a) 20769..20993 - 225 WP_019463222.1 type II toxin-antitoxin system VapB family antitoxin -
RMP42_RS19440 (RMP42_03205) 21106..22230 - 1125 WP_019463223.1 site-specific integrase -
RMP42_RS19445 (RMP42_03206b) 22227..22634 - 408 WP_019463224.1 PIN domain-containing protein Toxin
RMP42_RS19450 (RMP42_03207b) 22631..22867 - 237 WP_019463225.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RMP42_RS19455 (RMP42_03208) 23150..23923 + 774 WP_026033587.1 hypothetical protein -
RMP42_RS19465 (RMP42_03255) 25246..26199 - 954 WP_115359383.1 IS630 family transposase -
RMP42_RS19470 26294..27099 + 806 WP_085977442.1 IS5 family transposase -
RMP42_RS19475 (RMP42_03258) 27096..27734 - 639 WP_019463387.1 extracellular solute-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..385778 385778


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-115)

Antitoxin

SpoVT-AbrB domain

(5-36)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14660.95 Da        Isoelectric Point: 6.9526

>T116921 WP_019463224.1 NZ_CP034903:c22634-22227 [Roseomonas mucosa]
MNRPFLDTNILLYAALQPDPRSETARGLLARGGLVSVQVLNEFTAVARRKLRRPWPEVMQALAAIRTLCPAPRPLTVATH
EAAIGLAERLGYTIDNALILASALEAGCDTLFSEDLQDGQVVEGRLTIRNPFAAP

Download         Length: 408 bp

>T116921 NZ_CP034903:c22634-22227 [Roseomonas mucosa]
ATGAACCGGCCCTTCCTCGATACCAACATCCTGCTCTACGCCGCCCTGCAGCCGGATCCGCGTTCCGAGACGGCGCGCGG
CCTCCTGGCCCGGGGCGGCCTGGTCAGCGTCCAGGTGCTCAACGAGTTCACCGCCGTCGCCCGCCGCAAGCTGCGCCGGC
CCTGGCCCGAGGTCATGCAGGCGCTGGCCGCCATCCGGACCCTCTGTCCGGCGCCCCGGCCGCTCACCGTCGCGACGCAC
GAGGCGGCCATCGGGCTGGCGGAGAGGCTGGGCTACACGATCGACAACGCCCTGATCCTGGCCTCGGCCCTGGAGGCCGG
CTGCGACACGCTCTTCTCCGAGGATCTGCAGGACGGGCAGGTGGTCGAAGGGCGGCTGACCATCCGCAACCCCTTCGCCG
CGCCATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8666.71 Da        Isoelectric Point: 6.4685

>AT116921 WP_019463225.1 NZ_CP034903:c22867-22631 [Roseomonas mucosa]
MQVSRWGNSLAVRLPAAVVEALGLREGDEVAIEVAGPHTFRVARDRRRDEAIEALRGLGWHLPDGFRFSRDEANERGG

Download         Length: 237 bp

>AT116921 NZ_CP034903:c22867-22631 [Roseomonas mucosa]
ATGCAGGTCAGCCGCTGGGGCAATAGCCTCGCCGTCCGTCTCCCCGCCGCCGTGGTCGAGGCTTTGGGCCTGCGCGAAGG
CGACGAGGTCGCCATCGAGGTGGCCGGCCCCCACACCTTCCGCGTGGCCCGCGACCGCCGCCGCGACGAGGCGATCGAGG
CGCTGCGCGGTCTCGGCTGGCACCTGCCCGACGGCTTCCGCTTCTCGCGCGACGAGGCCAACGAGCGCGGCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A379PMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A379PNA3

References