Detailed information of TA system TA116644

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 376473..376698
• Replicon: chromosome
• Accession: NZ_CP034797
• Organism: Escherichia coli strain 08-3918

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: AYP37_RS02480
• Protein ID: WP_000813258.1
• Coordinates: 376473..376628 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	376640..376698 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP37_RS02430	371476..371907	-	432	WP_001302123.1	tellurite resistance protein	-
AYP37_RS02445	372358..373071	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
AYP37_RS02450	373207..373404	-	198	WP_000917763.1	hypothetical protein	-
AYP37_RS02455	373629..374183	-	555	WP_000640035.1	DUF1133 family protein	-
AYP37_RS02460	374246..374551	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
AYP37_RS02465	374564..375613	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
AYP37_RS02470	375615..375887	-	273	WP_000191871.1	hypothetical protein	-
AYP37_RS02475	376009..376353	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
AYP37_RS02480	376473..376628	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	376640..376698	+	59	-	-	Antitoxin
AYP37_RS02485	376919..377476	-	558	WP_000104474.1	DUF551 domain-containing protein	-
AYP37_RS02490	377478..377696	-	219	WP_000683609.1	DUF4014 family protein	-
AYP37_RS02495	377824..378135	-	312	WP_001289673.1	hypothetical protein	-
AYP37_RS02500	378128..378355	-	228	WP_000699809.1	hypothetical protein	-
AYP37_RS02505	378352..378633	-	282	WP_000603384.1	DNA-binding protein	-
AYP37_RS02510	378666..379382	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
AYP37_RS02515	379416..379877	-	462	WP_000139447.1	replication protein	-
AYP37_RS02520	379870..380925	-	1056	WP_001356791.1	hypothetical protein	-
AYP37_RS02525	380994..381419	-	426	WP_000693878.1	Rha family transcriptional regulator	-
AYP37_RS02530	381403..381646	-	244	Protein_376	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	339025..437187	98162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T116644 WP_000813258.1 NZ_CP034797:c376628-376473 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T116644 NZ_CP034797:c376628-376473 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT116644 NZ_CP034797:376640-376698 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin