Detailed information of TA system TA116347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 86251..87047
• Replicon: plasmid pPvSC3
• Accession: NZ_CP034667
• Organism: Proteus vulgaris strain PvSC3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A8I2DCD2
• Locus tag: EKQ45_RS00550
• Protein ID: WP_042847109.1
• Coordinates: 86535..87047 (+)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A8I2DCF1
• Locus tag: EKQ45_RS00545
• Protein ID: WP_042847112.1
• Coordinates: 86251..86538 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKQ45_RS00515	81509..81997	+	489	WP_166268109.1	hypothetical protein	-
EKQ45_RS00520	82104..82280	+	177	WP_162762197.1	hypothetical protein	-
EKQ45_RS00525	82340..82900	+	561	WP_052219368.1	hypothetical protein	-
EKQ45_RS00530	82897..83529	+	633	WP_052219367.1	hypothetical protein	-
EKQ45_RS00535	83809..84723	+	915	WP_042847118.1	sel1 repeat family protein	-
EKQ45_RS00540	84819..86105	+	1287	WP_042847114.1	sel1 repeat family protein	-
EKQ45_RS00545	86251..86538	+	288	WP_042847112.1	DUF1778 domain-containing protein	Antitoxin
EKQ45_RS00550	86535..87047	+	513	WP_042847109.1	GNAT family N-acetyltransferase	Toxin
EKQ45_RS00555	87135..87416	+	282	WP_042847108.1	hypothetical protein	-
EKQ45_RS00560	87457..87846	+	390	WP_042847106.1	hypothetical protein	-
EKQ45_RS00565	88452..89762	+	1311	Protein_112	NERD domain-containing protein	-
EKQ45_RS00570	89682..90743	-	1062	WP_191901264.1	hypothetical protein	-
EKQ45_RS00575	91223..91900	+	678	WP_191901265.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / floR / ant(2'')-Ia / catB8 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / tet(B) / cfr	-	1..284528	284528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (32-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-87)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 18357.30 Da
• Isoelectric Point: 9.5976

>T116347 WP_042847109.1 NZ_CP034667:86535-87047 [Proteus vulgaris]
MTLKLTAPEPLKTGHLLEGFSSGEEALDLWLKNRAMSNQNSGASRTFVTTSDNQVMGYYALSSGIVSTNQAVGRFRRNMP
SDIPVILLGRLAVDTRAKGLGVGRGLVKDACHRVIQASGLVGIRGIVVHALTDDAKRFYEHIGFVPSPLDPMMLMITLAD
LQLAMGIHPN

Nucleotide

• Length: 513 bp

>T116347 NZ_CP034667:86535-87047 [Proteus vulgaris]
ATGACATTGAAGCTGACAGCACCCGAACCCCTCAAAACAGGCCATTTGCTGGAAGGCTTTTCATCTGGTGAAGAGGCTTT
AGATCTCTGGCTAAAAAATCGGGCCATGAGTAATCAAAACTCAGGAGCTTCACGAACATTTGTAACCACTTCTGATAACC
AAGTGATGGGGTATTATGCCTTGTCTTCCGGGATTGTGAGCACCAATCAAGCTGTTGGCCGATTCCGCCGCAATATGCCT
TCTGACATACCAGTCATCCTTCTCGGTAGACTGGCAGTGGATACAAGAGCTAAAGGGCTTGGAGTTGGAAGGGGATTGGT
CAAAGATGCCTGTCATCGTGTTATTCAGGCCTCTGGGCTGGTGGGCATACGAGGAATAGTGGTCCACGCTCTAACTGATG
ATGCTAAACGCTTCTATGAACACATTGGTTTCGTCCCTTCACCACTTGACCCTATGATGCTAATGATAACACTCGCCGAT
CTGCAACTCGCAATGGGTATTCATCCAAACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10722.20 Da
• Isoelectric Point: 8.6145

>AT116347 WP_042847112.1 NZ_CP034667:86251-86538 [Proteus vulgaris]
MATQNDVAVTRSSLNLRIKPEDKILIDRAANAVGKNRTEFVLEAARRAAEETLADLRVINVSPEVYQEFINQLDAAPRNN
EALRKTLMSKSPWEK

Nucleotide

• Length: 288 bp

>AT116347 NZ_CP034667:86251-86538 [Proteus vulgaris]
ATGGCAACTCAAAATGATGTAGCGGTAACACGCTCATCTCTGAACCTGAGAATTAAACCGGAAGACAAAATCCTGATCGA
CCGCGCCGCAAATGCGGTAGGCAAAAATCGAACTGAGTTCGTTCTGGAAGCCGCTCGACGAGCTGCTGAAGAGACACTGG
CGGACCTGCGGGTTATTAACGTCAGCCCCGAGGTATACCAGGAGTTTATTAATCAGCTTGATGCCGCACCAAGGAACAAT
GAGGCATTACGAAAAACATTGATGTCTAAATCCCCTTGGGAGAAATGA