Detailed information of TA system TA116135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 89607..90437
• Replicon: chromosome
• Accession: NZ_CP034482
• Organism: Rahnella aquatilis strain KM12

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: EJP81_RS00430
• Protein ID: WP_126124564.1
• Coordinates: 89607..90086 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0H3F4N7
• Locus tag: EJP81_RS00435
• Protein ID: WP_013573435.1
• Coordinates: 90093..90437 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP81_RS00405	84892..86337	+	1446	WP_112151980.1	alpha/beta hydrolase	-
EJP81_RS00410	86408..86866	+	459	WP_013573431.1	MarR family transcriptional regulator	-
EJP81_RS00415	87001..87423	+	423	WP_013573432.1	organic hydroperoxide resistance protein	-
EJP81_RS00420	87695..89374	+	1680	WP_013573433.1	kdo(2)-lipid A phosphoethanolamine 7''-transferase	-
EJP81_RS00430	89607..90086	-	480	WP_126124564.1	type II toxin-antitoxin system YhaV family toxin	Toxin
EJP81_RS00435	90093..90437	-	345	WP_013573435.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
EJP81_RS00440	90519..90680	-	162	WP_071882947.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EJP81_RS00445	90821..92200	-	1380	WP_013573436.1	MmgE/PrpD family protein	-
EJP81_RS00450	92220..93002	-	783	WP_014411455.1	ABC transporter ATP-binding protein	-
EJP81_RS00455	93002..93868	-	867	WP_014411456.1	ABC transporter ATP-binding protein	-
EJP81_RS00460	93861..94796	-	936	WP_112197519.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (13-148)

Antitoxin

• SpoVT-AbrB domain (15-53)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 18699.55 Da
• Isoelectric Point: 9.9550

>T116135 WP_126124564.1 NZ_CP034482:c90086-89607 [Rahnella aquatilis]
MHNQAMEINGWTLLAHPLILQQIQALTDQVVLLRRKDPAGYKSKNATKRLAAINKLMFDVIPQDPARVEFRQGDTLGGEF
SHWFRAKFFQQYRLFFRYNLKSRIIIYAWVNDDKELRAYESKTDAYRMFRKMLKSGEPPDNWQALCEASGKIMAQEEQP

Nucleotide

• Length: 480 bp

>T116135 NZ_CP034482:c90086-89607 [Rahnella aquatilis]
ATGCACAATCAGGCCATGGAGATTAACGGATGGACCCTTCTCGCTCATCCTCTGATCCTGCAACAAATACAGGCACTCAC
TGATCAGGTTGTTTTACTGCGCCGCAAAGATCCGGCGGGCTATAAAAGTAAAAATGCCACCAAACGGCTGGCTGCGATCA
ATAAGCTGATGTTTGACGTTATCCCGCAGGATCCGGCGCGTGTTGAATTCCGTCAGGGCGATACGTTAGGCGGGGAATTC
TCACACTGGTTCAGGGCAAAATTCTTCCAGCAGTACCGTTTGTTTTTCCGCTACAACCTGAAAAGCCGGATCATTATTTA
CGCCTGGGTGAATGATGATAAAGAATTACGCGCCTACGAAAGCAAAACAGACGCATACCGGATGTTTCGCAAGATGTTGA
AAAGCGGTGAACCGCCAGACAACTGGCAGGCACTTTGCGAGGCGTCAGGGAAAATAATGGCTCAGGAAGAACAGCCGTAG

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12701.48 Da
• Isoelectric Point: 4.3324

>AT116135 WP_013573435.1 NZ_CP034482:c90437-90093 [Rahnella aquatilis]
MSASALWGIKFEAESKVTRRFQTTIPATIRKALNLTENDRIQYKILPDGQVVISRQLEEAEDPVISAFLGFVAKDMLNNP
ENMQPVTLSLHEKINVLTAGMAIDLESPLSDDDE

Nucleotide

• Length: 345 bp

>AT116135 NZ_CP034482:c90437-90093 [Rahnella aquatilis]
ATGTCAGCCTCGGCACTATGGGGAATAAAATTCGAAGCCGAATCCAAAGTCACCCGCCGCTTCCAGACCACCATTCCGGC
CACTATCCGTAAGGCATTAAATCTCACCGAAAATGATCGGATCCAGTACAAGATTTTGCCAGACGGGCAGGTGGTTATTT
CGAGGCAACTCGAAGAAGCGGAAGACCCTGTTATCAGTGCTTTTCTCGGTTTTGTGGCAAAAGATATGCTCAATAACCCG
GAAAATATGCAGCCGGTAACATTATCCCTGCATGAAAAAATCAATGTACTGACCGCCGGGATGGCTATCGATCTTGAGAG
CCCGCTTTCTGACGATGACGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3F4N7