Detailed information of TA system TA116071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1917677..1918485
• Replicon: chromosome
• Accession: NZ_CP034447
• Organism: Mesorhizobium sp. M1E.F.Ca.ET.045.02.1.1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EJ070_RS09115
• Protein ID: WP_126091051.1
• Coordinates: 1917964..1918485 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: EJ070_RS09110
• Protein ID: WP_126091050.1
• Coordinates: 1917677..1917967 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ070_RS09075	1912923..1913744	+	822	WP_126091045.1	4-hydroxy-tetrahydrodipicolinate reductase	-
EJ070_RS09080	1913836..1914456	+	621	WP_126091046.1	2,3-bisphosphoglycerate-dependent phosphoglycerate mutase	-
EJ070_RS09095	1915027..1916709	+	1683	WP_126091047.1	FAD-dependent oxidoreductase	-
EJ070_RS09100	1916709..1917014	+	306	WP_126091048.1	UBP-type zinc finger domain-containing protein	-
EJ070_RS09105	1917119..1917310	-	192	WP_126091049.1	Flp family type IVb pilin	-
EJ070_RS09110	1917677..1917967	+	291	WP_126091050.1	DUF1778 domain-containing protein	Antitoxin
EJ070_RS09115	1917964..1918485	+	522	WP_126091051.1	GNAT family N-acetyltransferase	Toxin
EJ070_RS36220	1918515..1918688	-	174	WP_189350454.1	hypothetical protein	-
EJ070_RS09120	1919011..1919838	+	828	WP_126091052.1	glycosyltransferase family 2 protein	-
EJ070_RS09125	1919872..1920678	-	807	WP_126091053.1	aspartate/glutamate racemase family protein	-
EJ070_RS09130	1920736..1921977	-	1242	WP_126091054.1	D-amino acid dehydrogenase	-
EJ070_RS09135	1922561..1923289	-	729	WP_126091055.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-95)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 18525.14 Da
• Isoelectric Point: 9.8812

>T116071 WP_126091051.1 NZ_CP034447:1917964-1918485 [Mesorhizobium sp. M1E.F.Ca.ET.045.02.1.1]
VTLQAPVPLDRGHDLSSFSSGIPASDDWVKRRALANQDSGATRTFVTCKDDKVVAYYALASGGVIASHVPGRFRRNMPDP
IPVAILARLAIDTSCQRQGIGRALVQDAARRVMNAAGSLGIRGILVHAISDEARSYYTALGFDPSPRDPMTLVISLADLR
EAIRPKEPTSKSG

Nucleotide

• Length: 522 bp

>T116071 NZ_CP034447:1917964-1918485 [Mesorhizobium sp. M1E.F.Ca.ET.045.02.1.1]
GTGACGCTCCAGGCGCCGGTACCGCTGGATCGTGGCCACGACCTTTCGTCTTTTTCATCCGGCATTCCGGCGTCGGATGA
CTGGGTCAAACGCCGAGCGCTCGCCAACCAGGACAGTGGCGCGACACGCACCTTCGTCACTTGCAAGGACGACAAGGTTG
TCGCCTACTACGCGCTCGCTTCGGGCGGCGTCATTGCCAGCCATGTGCCAGGCCGGTTTCGGCGCAACATGCCCGATCCG
ATTCCGGTAGCAATTCTTGCTCGTCTCGCAATCGACACTTCCTGTCAGCGACAAGGAATCGGCAGGGCGCTCGTCCAGGA
TGCAGCGCGCAGGGTGATGAATGCTGCAGGGTCGCTCGGCATCCGCGGCATCTTGGTCCACGCCATCTCGGATGAAGCGC
GAAGCTACTATACGGCGCTTGGGTTCGACCCCTCGCCTCGCGATCCCATGACGCTGGTGATCTCTCTCGCCGATCTGCGT
GAGGCGATCAGACCAAAGGAACCAACCAGCAAAAGCGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10919.64 Da
• Isoelectric Point: 8.5998

>AT116071 WP_126091050.1 NZ_CP034447:1917677-1917967 [Mesorhizobium sp. M1E.F.Ca.ET.045.02.1.1]
MKHEPPDASKRSTLNMRIKPEERGLIDEAARTLGKTRTDFILDAARRMAEDTLLERTLIKVSPEAYAEFLALLDVPAKSN
ERLSKLMNAPLPWETK

Nucleotide

• Length: 291 bp

>AT116071 NZ_CP034447:1917677-1917967 [Mesorhizobium sp. M1E.F.Ca.ET.045.02.1.1]
ATGAAGCACGAACCTCCAGACGCTTCCAAGAGAAGCACGCTGAACATGCGCATCAAGCCCGAGGAGCGCGGCTTGATCGA
CGAAGCCGCGCGGACGCTCGGCAAGACCCGGACGGATTTCATCCTCGACGCTGCCCGGCGTATGGCTGAGGACACGCTGC
TCGAGCGCACGCTTATCAAGGTCTCGCCGGAAGCCTATGCCGAATTCCTTGCTCTGCTCGATGTGCCGGCCAAGTCGAAC
GAGCGACTGTCCAAACTGATGAACGCGCCACTGCCATGGGAAACTAAGTGA