Detailed information of TA system TA116003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1228..1702
• Replicon: plasmid unnamed2
• Accession: NZ_CP034419
• Organism: Klebsiella pneumoniae strain C789

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: EJJ25_RS28690
• Protein ID: WP_004197762.1
• Coordinates: 1436..1702 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: EJJ25_RS28685
• Protein ID: WP_000879771.1
• Coordinates: 1228..1452 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJJ25_RS28680	349..561	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
EJJ25_RS28685	1228..1452	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
EJJ25_RS28690	1436..1702	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJJ25_RS28695	1772..2122	-	351	WP_004197756.1	hypothetical protein	-
EJJ25_RS28700	2188..2733	-	546	WP_023317533.1	hypothetical protein	-
EJJ25_RS28705	2759..3138	-	380	Protein_5	hypothetical protein	-
EJJ25_RS30320	4241..4417	+	177	WP_004197757.1	hypothetical protein	-
EJJ25_RS28710	4407..4751	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
EJJ25_RS28715	4741..5589	+	849	Protein_8	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T116003 WP_004197762.1 NZ_CP034419:1436-1702 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T116003 NZ_CP034419:1436-1702 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT116003 WP_000879771.1 NZ_CP034419:1228-1452 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT116003 NZ_CP034419:1228-1452 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5