Detailed information of TA system TA115919

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 446716..447560
• Replicon: chromosome
• Accession: NZ_CP034404
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: EJC75_RS03985
• Protein ID: WP_000854686.1
• Coordinates: 447177..447560 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: EJC75_RS03980
• Protein ID: WP_001285602.1
• Coordinates: 446716..447096 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS03940	442964..443419	+	456	WP_000581506.1	hypothetical protein	-
EJC75_RS03945	443520..443728	+	209	Protein_401	DUF905 family protein	-
EJC75_RS03955	443829..444650	+	822	WP_001761104.1	DUF945 domain-containing protein	-
EJC75_RS03960	444742..445227	+	486	WP_000214307.1	antirestriction protein	-
EJC75_RS03965	445243..445719	+	477	WP_129484016.1	RadC family protein	-
EJC75_RS03970	445782..446003	+	222	WP_000692298.1	DUF987 domain-containing protein	-
EJC75_RS03975	446022..446705	+	684	WP_000086768.1	hypothetical protein	-
EJC75_RS03980	446716..447096	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EJC75_RS03985	447177..447560	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
EJC75_RS03990	447557..448045	+	489	WP_032180032.1	hypothetical protein	-
EJC75_RS03995	448062..448259	+	198	WP_000839253.1	DUF957 domain-containing protein	-
EJC75_RS04000	448344..449185	+	842	Protein_411	DUF4942 domain-containing protein	-
EJC75_RS04010	449656..450594	+	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
EJC75_RS04015	450649..451386	+	738	WP_000283664.1	zinc-binding phosphatase	-
EJC75_RS04020	451410..451960	+	551	Protein_414	molecular chaperone YcdY	-
EJC75_RS04025	452062..452553	+	492	WP_001297187.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T115919 WP_000854686.1 NZ_CP034404:447177-447560 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T115919 NZ_CP034404:447177-447560 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT115919 WP_001285602.1 NZ_CP034404:446716-447096 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT115919 NZ_CP034404:446716-447096 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG