Detailed information of TA system TA115864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2379010..2379677
• Replicon: chromosome
• Accession: NZ_CP034395
• Organism: Herbaspirillum seropedicae strain AU13965

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EJD96_RS10940
• Protein ID: WP_139970897.1
• Coordinates: 2379261..2379677 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EJD96_RS10935
• Protein ID: WP_013234200.1
• Coordinates: 2379010..2379264 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJD96_RS10925	2375655..2377259	-	1605	WP_139970895.1	L-aspartate oxidase	-
EJD96_RS10930	2377426..2378637	-	1212	WP_139970896.1	formate-dependent phosphoribosylglycinamide formyltransferase	-
EJD96_RS10935	2379010..2379264	+	255	WP_013234200.1	plasmid stabilization protein	Antitoxin
EJD96_RS10940	2379261..2379677	+	417	WP_139970897.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJD96_RS10945	2379702..2380004	-	303	WP_139970898.1	hypothetical protein	-
EJD96_RS10950	2380006..2381361	-	1356	WP_139970899.1	DUF1446 domain-containing protein	-
EJD96_RS10955	2381404..2382705	-	1302	WP_139970900.1	citrate:proton symporter	-
EJD96_RS10960	2382889..2383905	+	1017	WP_139970901.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14959.25 Da
• Isoelectric Point: 5.1280

>T115864 WP_139970897.1 NZ_CP034395:2379261-2379677 [Herbaspirillum seropedicae]
MIVLDTNVVSEAMKPAPDAAVQAWFNDQAPETLYLTSITVAEIGFGIACLSAGRRQDLLARRFEGLLRMFKDRILPFDMD
AARSHAELAAMARRAGRGFPTPDGYIAAIAASCGFLVASRDVTPYLAAELTVINPWQA

Nucleotide

• Length: 417 bp

>T115864 NZ_CP034395:2379261-2379677 [Herbaspirillum seropedicae]
ATGATCGTCCTGGATACCAATGTGGTGTCCGAGGCCATGAAGCCGGCCCCGGACGCCGCCGTACAGGCCTGGTTCAATGA
CCAGGCGCCCGAGACGCTCTACCTGACCAGCATTACGGTGGCCGAGATCGGCTTCGGCATAGCTTGCCTGTCTGCTGGCC
GTCGCCAGGATTTGCTGGCGCGTCGCTTCGAGGGCTTGCTGCGCATGTTCAAGGACCGCATCCTGCCCTTCGATATGGAC
GCCGCACGCTCGCATGCCGAGCTGGCCGCCATGGCCAGACGTGCAGGCCGGGGATTTCCCACGCCGGATGGCTACATAGC
CGCCATTGCCGCTTCCTGCGGATTCCTCGTGGCGTCGCGCGATGTCACCCCTTATCTGGCCGCAGAGCTGACGGTCATCA
ATCCCTGGCAGGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9288.55 Da
• Isoelectric Point: 6.4677

>AT115864 WP_013234200.1 NZ_CP034395:2379010-2379264 [Herbaspirillum seropedicae]
MATLTVRNLPDEVHRALRVRAAQHGRSTEAEIREILTLAVRPAGQLRLGDALAAMSRELGLSNEDVEALQQTRERAPAQP
MSFE

Nucleotide

• Length: 255 bp

>AT115864 NZ_CP034395:2379010-2379264 [Herbaspirillum seropedicae]
ATGGCCACCCTCACAGTCCGGAACCTCCCCGACGAGGTCCATCGTGCCCTGCGCGTGCGCGCGGCCCAGCATGGCCGCAG
CACCGAGGCGGAAATCCGCGAGATTCTCACCCTGGCAGTCAGGCCTGCCGGACAACTGCGCCTGGGAGACGCGCTGGCGG
CAATGAGCCGAGAGCTGGGTCTGAGCAATGAAGATGTCGAAGCCCTGCAGCAGACCCGCGAACGTGCGCCAGCCCAGCCG
ATGAGTTTCGAATGA