Detailed information of TA system TA115690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 66893..67461
• Replicon: plasmid pVPCZ2014-1
• Accession: NZ_CP034291
• Organism: Vibrio parahaemolyticus strain 20140722001-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: EHC64_RS26185
• Protein ID: WP_023624650.1
• Coordinates: 66893..67234 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EHC64_RS26190
• Protein ID: WP_017449238.1
• Coordinates: 67231..67461 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC64_RS26160	61981..62619	+	639	Protein_57	IS5 family transposase	-
EHC64_RS26165	62953..64041	+	1089	WP_086370877.1	replication initiation protein	-
EHC64_RS26175	65284..66015	+	732	WP_025789518.1	AHH domain-containing protein	-
EHC64_RS26180	66015..66590	+	576	WP_017449240.1	hypothetical protein	-
EHC64_RS26185	66893..67234	-	342	WP_023624650.1	endoribonuclease MazF	Toxin
EHC64_RS26190	67231..67461	-	231	WP_017449238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHC64_RS26195	67912..68637	+	726	WP_017449237.1	site-specific integrase	-
EHC64_RS26200	68632..69015	-	384	Protein_64	recombinase family protein	-
EHC64_RS26205	69618..69839	-	222	WP_023624649.1	hypothetical protein	-
EHC64_RS26210	70149..70523	+	375	WP_127914005.1	hypothetical protein	-
EHC64_RS26215	71084..72115	+	1032	WP_159408187.1	Ig-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105369	105369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T115690 WP_023624650.1 NZ_CP034291:c67234-66893 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T115690 NZ_CP034291:c67234-66893 [Vibrio parahaemolyticus]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGAGTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGGTATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGAGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTAGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT115690 WP_017449238.1 NZ_CP034291:c67461-67231 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT115690 NZ_CP034291:c67461-67231 [Vibrio parahaemolyticus]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGCTTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95