Detailed information of TA system TA115563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4198974..4199758
• Replicon: chromosome
• Accession: NZ_CP034236
• Organism: Pectobacterium carotovorum subsp. carotovorum strain BP201601.1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EH203_RS18820
• Protein ID: WP_029368386.1
• Coordinates: 4198974..4199468 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A855MHZ3
• Locus tag: EH203_RS18825
• Protein ID: WP_010301483.1
• Coordinates: 4199465..4199758 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH203_RS18805	4194484..4196763	-	2280	WP_010301495.1	S8 family peptidase	-
EH203_RS18810	4196766..4197890	-	1125	WP_005970980.1	ATP-binding protein	-
EH203_RS18815	4198742..4198912	-	171	Protein_3683	helix-turn-helix domain-containing protein	-
EH203_RS18820	4198974..4199468	-	495	WP_029368386.1	GNAT family N-acetyltransferase	Toxin
EH203_RS18825	4199465..4199758	-	294	WP_010301483.1	DUF1778 domain-containing protein	Antitoxin
EH203_RS18830	4200087..4200989	-	903	WP_029368384.1	LysR family transcriptional regulator	-
EH203_RS18835	4201118..4201381	+	264	WP_010286385.1	DUF2798 domain-containing protein	-
EH203_RS18840	4201474..4202259	-	786	WP_180890064.1	phosphonate metabolism protein PhnP	-
EH203_RS18845	4202298..4202867	-	570	WP_161502897.1	ribose 1,5-bisphosphokinase	-
EH203_RS18850	4202986..4203198	-	213	Protein_3690	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
EH203_RS18855	4203195..4203416	-	222	Protein_3691	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17583.40 Da
• Isoelectric Point: 7.8050

>T115563 WP_029368386.1 NZ_CP034236:c4199468-4198974 [Pectobacterium carotovorum subsp. carotovorum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARNFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T115563 NZ_CP034236:c4199468-4198974 [Pectobacterium carotovorum subsp. carotovorum]
ATGATCTCAGCCCCTGAGCCACTCCACGCCGAACATGTACTTTCCTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGTGCATCGCGAACTTTTGTCAGTTGCGATGGCTCAAAAGTGCTGG
CCTATTACTCATTGGCCTCCAGTGCTGTAGCAACGAATGCTGCCCCCGGACGTTTTCGCCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTGGATACGTCATTGCATGGTCAAGGTGTTGGCCGAGCGCTGGTGCGTGATGC
CGGGCTTCGGGTTATTCAGGTAGCGGACACTATCGGCATTCGCGGGATGTTGGTTCATGCGTTGTCTGATGAAGCGCGAA
ATTTTTACCTACGGGTGGGGTTTGAGCCTTCACCGATTGATCCCATGATGTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10930.52 Da
• Isoelectric Point: 10.1968

>AT115563 WP_010301483.1 NZ_CP034236:c4199758-4199465 [Pectobacterium carotovorum subsp. carotovorum]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQTPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT115563 NZ_CP034236:c4199758-4199465 [Pectobacterium carotovorum subsp. carotovorum]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTAAATTTACGCATCAAGCCCGCCGAACGAGATCTTATTGA
TCGTGCTGCGAAGGCCAGAGGAAAAAATCGCACTGATTTTGTCCTCGAAGCTGCTCGTGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAATTCCTTGCTCGCTTGGATCAAACTCCCTCGCCGAAT
GCCGCGCTGCGCAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A855MHZ3