Detailed information of TA system TA115506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4504418..4505008
• Replicon: chromosome
• Accession: NZ_CP034212
• Organism: Escherichia albertii strain 2010C-3449

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: CCF17_RS23050
• Protein ID: WP_059225520.1
• Coordinates: 4504676..4505008 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: CCF17_RS23045
• Protein ID: WP_059225519.1
• Coordinates: 4504418..4504675 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF17_RS23010 (4501261)	4501261..4501431	-	171	Protein_4401	Arm DNA-binding domain-containing protein	-
CCF17_RS23020 (4501993)	4501993..4502427	+	435	Protein_4402	LysR family transcriptional regulator	-
CCF17_RS23025 (4502545)	4502545..4503078	+	534	Protein_4403	LysR substrate-binding domain-containing protein	-
CCF17_RS23030 (4503143)	4503143..4503840	+	698	WP_225520032.1	IS1-like element IS1A family transposase	-
CCF17_RS23035 (4503865)	4503865..4504070	+	206	Protein_4405	helix-turn-helix transcriptional regulator	-
CCF17_RS23045 (4504418)	4504418..4504675	+	258	WP_059225519.1	hypothetical protein	Antitoxin
CCF17_RS23050 (4504676)	4504676..4505008	+	333	WP_059225520.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CCF17_RS25845 (4505120)	4505120..4505497	+	378	WP_241201721.1	hypothetical protein	-
CCF17_RS23070 (4505815)	4505815..4506699	+	885	WP_151402214.1	integrase domain-containing protein	-
CCF17_RS23075 (4506795)	4506795..4507082	-	288	WP_151402215.1	hypothetical protein	-
CCF17_RS23080 (4507807)	4507807..4509069	-	1263	WP_151402216.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rhs/PAAR	4494379..4560379	66000
	-	flank	IS/Tn	-	-	4503337..4503840	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11777.59 Da
• Isoelectric Point: 8.0291

>T115506 WP_059225520.1 NZ_CP034212:4504676-4505008 [Escherichia albertii]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSPASFNEVTRLPVIVPVTSGGQFARSAGFAVSLEGAGTKTTGIIRCDQPRTI
DMGARNGKRLERIPDGIINEVLARLETILA

Nucleotide

• Length: 333 bp

>T115506 NZ_CP034212:4504676-4505008 [Escherichia albertii]
ATGGACAGAGGAGAAATCTGGCTTGTTTCACTTGACCCTATCGCCGGGCATGAGCAAAGCGGAAAACGCCCGGTGCTTAT
CGTTTCTCCGGCCTCGTTTAACGAGGTCACCCGGTTGCCCGTCATTGTTCCGGTAACCAGCGGTGGGCAATTTGCCCGTA
GCGCGGGTTTTGCGGTTTCGCTGGAGGGCGCTGGCACAAAAACGACGGGGATTATTCGTTGCGATCAGCCCAGAACCATT
GATATGGGAGCCCGAAACGGTAAGCGGCTGGAGCGCATACCTGACGGTATCATCAACGAGGTGCTGGCAAGGCTGGAAAC
CATCCTGGCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9314.80 Da
• Isoelectric Point: 4.4488

>AT115506 WP_059225519.1 NZ_CP034212:4504418-4504675 [Escherichia albertii]
MYTTRLKKVGGSVMLAVPPAVLKTLALLPDSEVGVTIDNGCLIIEPQKRPRYSLAELLAQCDPHEEISDEEREWIDAPAA
GKEIL

Nucleotide

• Length: 258 bp

>AT115506 NZ_CP034212:4504418-4504675 [Escherichia albertii]
ATGTATACCACACGTCTGAAAAAGGTTGGCGGTTCTGTCATGCTGGCTGTTCCGCCCGCCGTGCTGAAAACGCTGGCCCT
GTTGCCGGACAGCGAAGTCGGTGTAACCATCGACAACGGCTGCCTGATTATCGAACCGCAAAAACGACCACGCTATTCAC
TGGCCGAACTGCTGGCGCAATGCGATCCCCATGAAGAAATCAGTGATGAGGAGCGCGAATGGATTGATGCGCCTGCCGCA
GGGAAGGAGATCCTGTAA