Detailed information of TA system TA115380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 165744..166565
• Replicon: plasmid pPagL15_1
• Accession: NZ_CP034149
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CBF16_RS19890
• Protein ID: WP_010247344.1
• Coordinates: 166095..166565 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: CBF16_RS19885
• Protein ID: WP_033773852.1
• Coordinates: 165744..166088 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS19865	161739..162854	+	1116	WP_124890532.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
CBF16_RS19870	162898..164199	+	1302	WP_033760237.1	maltoporin	-
CBF16_RS19875	164302..165240	+	939	WP_124890533.1	maltose operon protein MalM	-
CBF16_RS19880	165429..165644	+	216	WP_061703419.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CBF16_RS19885	165744..166088	+	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
CBF16_RS19890	166095..166565	+	471	WP_010247344.1	type II toxin-antitoxin system YhaV family toxin	Toxin
CBF16_RS19895	166755..168830	-	2076	WP_124890534.1	4-alpha-glucanotransferase	-
CBF16_RS19900	168839..171241	-	2403	WP_124890535.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..583567	583567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18278.80 Da
• Isoelectric Point: 8.9277

>T115380 WP_010247344.1 NZ_CP034149:166095-166565 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQDWANTNS

Nucleotide

• Length: 471 bp

>T115380 NZ_CP034149:166095-166565 [Pantoea agglomerans]
ATGGAAGACAGCACGGTTATAAATGGATGGCACGTTTATGTTCATCCCTGTTTTGAATCGCAGCTGATTGCGCTGACCAA
TGAAGTGGAGTTACTCAGGGCTAAATATCCTGAAAGTTACCAGCGAAAAGCGACGACTAAGCTTTTAGCCGCTGTGTTTA
AAGTTATCAATAGTGAGATTTGCGCCGATCCACATCAGGCAAAGTTTCGCCAGGGCGATACGCTGGGAGAACAAAATAAG
CATTGGTTCCGTGCAAAGTTTTTGATGCAGTATCGTTTGTTCTTTAGATTCAGCGAGCAACATAAAACCATCATCCTTGC
ATGGATGAATGACGCTGAGACCAAACGTGCTTATGGCAGTAAGCGTGACGCCTATAAAGTATTTTCAGGAATGTTGCATA
ACGGCTATCCACCTGACGACTGGGCATTGCTGTTAAAGCAATCTCAGGATTGGGCGAATACAAATTCTTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT115380 WP_033773852.1 NZ_CP034149:165744-166088 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT115380 NZ_CP034149:165744-166088 [Pantoea agglomerans]
ATGGGAACTCTGACTCATGCAAACATCTCTCTGCGTGCTTCATCAAAACTGACAGCGCGCAGCCAGACCACTATCCCTGC
CGCAGTTCGTGATGCGATGAATCTGCAGCCTGGTGAGAATATCGAATACGCTGTTCTGCCGGGCGGCAAAGTCCTTATTA
CCCGTCAGGAAACGGTGACTGATGATGATCCAGTGATGGCGAGCTTTCTGAGTTTTCTTGCTGCTGACATACGCAACACA
CCTGCAAATATTAAAACGCTGGATCGAGGATTATTAACTCGCATTACATCGTTGACCGAAGGTATGGACGTTGATTTGGA
TGCTCCACTCATGGACGATGAGTAA