Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1518991..1519832 Replicon chromosome
Accession NZ_CP034106
Organism Escherichia coli strain RCAD0514

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag EGA47_RS07225 Protein ID WP_000854822.1
Coordinates 1518991..1519374 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A243ZR83
Locus tag EGA47_RS07230 Protein ID WP_071587515.1
Coordinates 1519464..1519832 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA47_RS07200 1514376..1516627 - 2252 Protein_1389 hypothetical protein -
EGA47_RS07210 1517361..1518206 - 846 WP_001280435.1 DUF4942 domain-containing protein -
EGA47_RS07215 1518303..1518500 - 198 WP_000839263.1 DUF957 domain-containing protein -
EGA47_RS07220 1518512..1519000 - 489 WP_001177592.1 hypothetical protein -
EGA47_RS07225 1518991..1519374 - 384 WP_000854822.1 type IV toxin-antitoxin system toxin CbtA Toxin
EGA47_RS07230 1519464..1519832 - 369 WP_071587515.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EGA47_RS07235 1519912..1520133 - 222 WP_000692312.1 DUF987 domain-containing protein -
EGA47_RS07240 1520202..1520678 - 477 WP_001186740.1 RadC family protein -
EGA47_RS07245 1520694..1521179 - 486 WP_063097556.1 antirestriction protein -
EGA47_RS07250 1521270..1522088 - 819 WP_001234702.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T115251 WP_000854822.1 NZ_CP034106:c1519374-1518991 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T115251 NZ_CP034106:c1519374-1518991 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13631.41 Da        Isoelectric Point: 6.6244

>AT115251 WP_071587515.1 NZ_CP034106:c1519832-1519464 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT115251 NZ_CP034106:c1519832-1519464 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A243ZR83

References