Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1110463..1111097 Replicon chromosome
Accession NZ_CP034078
Organism Pseudomonas syringae pv. pisi str. PP1

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag N032_RS05335 Protein ID WP_003345997.1
Coordinates 1110463..1110867 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M4XXE1
Locus tag N032_RS05340 Protein ID WP_003345999.1
Coordinates 1110867..1111097 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N032_RS05305 1105483..1105932 - 450 WP_024649228.1 DUF2384 domain-containing protein -
N032_RS05310 1106198..1107202 + 1005 WP_004419200.1 response regulator -
N032_RS05315 1107206..1107688 + 483 WP_024649227.1 PAS domain-containing protein -
N032_RS05320 1107785..1109041 + 1257 WP_004402530.1 3-phosphoshikimate 1-carboxyvinyltransferase -
N032_RS05325 1109064..1109726 - 663 WP_003408532.1 ChrR family anti-sigma-E factor -
N032_RS05330 1109726..1110238 - 513 WP_003345995.1 sigma-70 family RNA polymerase sigma factor -
N032_RS05335 1110463..1110867 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
N032_RS05340 1110867..1111097 - 231 WP_003345999.1 antitoxin Antitoxin
N032_RS05345 1111214..1112095 - 882 WP_024649226.1 aldose 1-epimerase -
N032_RS05350 1112070..1113041 - 972 WP_024649225.1 DMT family transporter -
N032_RS05355 1113126..1114406 - 1281 WP_003421655.1 TRAP transporter large permease -
N032_RS05360 1114407..1114934 - 528 WP_024649224.1 TRAP transporter small permease -
N032_RS05365 1114998..1116023 - 1026 WP_024649223.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T115151 WP_003345997.1 NZ_CP034078:c1110867-1110463 [Pseudomonas syringae pv. pisi str. PP1]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T115151 NZ_CP034078:c1110867-1110463 [Pseudomonas syringae pv. pisi str. PP1]
ATGCTCAAGTACATGCTTGACACCAATATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCATGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCCG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGGGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATCACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTACGAGTAGAAGACTGGGTAAGCC
AGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8580.59 Da        Isoelectric Point: 4.5557

>AT115151 WP_003345999.1 NZ_CP034078:c1111097-1110867 [Pseudomonas syringae pv. pisi str. PP1]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPAEQQRESL

Download         Length: 231 bp

>AT115151 NZ_CP034078:c1111097-1110867 [Pseudomonas syringae pv. pisi str. PP1]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTCGCCTTGCCAGACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCCGCCGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGTGAACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SM35

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4XXE1

References