Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 166852..167609 Replicon plasmid unnamed1
Accession NZ_CP034059
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag EGX88_RS01150 Protein ID WP_000501974.1
Coordinates 167124..167609 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag EGX88_RS01145 Protein ID WP_011114751.1
Coordinates 166852..167136 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS01110 (EGX88_01115) 162913..163587 + 675 WP_001088287.1 IS66-like element accessory protein TnpA -
EGX88_RS01115 (EGX88_01120) 163584..163934 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
EGX88_RS01120 (EGX88_01125) 163931..165532 + 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
EGX88_RS25555 165786..165950 - 165 WP_001346193.1 hypothetical protein -
EGX88_RS01145 (EGX88_01150) 166852..167136 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
EGX88_RS01150 (EGX88_01155) 167124..167609 + 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
EGX88_RS01155 (EGX88_01160) 167782..168063 - 282 Protein_207 IS4/IS5 family transposase -
EGX88_RS01160 (EGX88_01165) 168130..169286 + 1157 WP_134796931.1 IS3-like element IS600 family transposase -
EGX88_RS01165 (EGX88_01170) 169400..170628 + 1229 WP_094081542.1 IS3-like element IS2 family transposase -
EGX88_RS01170 (EGX88_01175) 170666..170809 + 144 Protein_210 DUF3440 domain-containing protein -
EGX88_RS01175 (EGX88_01180) 170794..171432 + 639 WP_000502862.1 ParB N-terminal domain-containing protein -
EGX88_RS01180 171660..172084 + 425 Protein_212 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospE2 / ipaH2.5 1..217451 217451
- inside IScluster/Tn - ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 154923..196226 41303


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T115071 WP_000501974.1 NZ_CP034059:167124-167609 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T115071 NZ_CP034059:167124-167609 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT115071 WP_011114751.1 NZ_CP034059:166852-167136 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT115071 NZ_CP034059:166852-167136 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References