Detailed information of TA system TA114571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1301..1848
• Replicon: plasmid p50579417_2
• Accession: NZ_CP033883
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EGM66_RS01675
• Protein ID: WP_025750222.1
• Coordinates: 1301..1582 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: EGM66_RS01680
• Protein ID: WP_000079928.1
• Coordinates: 1579..1848 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS01665	170..787	+	618	WP_025750220.1	hypothetical protein	-
EGM66_RS01675	1301..1582	-	282	WP_025750222.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGM66_RS01680	1579..1848	-	270	WP_000079928.1	hypothetical protein	Antitoxin
EGM66_RS28020	1903..2061	-	159	WP_032151498.1	hypothetical protein	-
EGM66_RS01695	2756..3628	-	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
EGM66_RS01710	4331..4972	+	642	Protein_5	transcription termination factor NusG	-
EGM66_RS01715	5112..5774	+	663	WP_005082527.1	hypothetical protein	-
EGM66_RS27920	6041..6184	+	144	WP_000176500.1	hypothetical protein	-
EGM66_RS01725	6265..6507	+	243	WP_021515452.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..88005	88005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10875.53 Da
• Isoelectric Point: 8.4615

>T114571 WP_025750222.1 NZ_CP033883:c1582-1301 [Escherichia coli]
MIEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T114571 NZ_CP033883:c1582-1301 [Escherichia coli]
ATGATAGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCGATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTACAAAGGTCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGTAAAGTG
TATATTTTGCGCGTGTTGCATACAGCACAGAAATGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT114571 WP_000079928.1 NZ_CP033883:c1848-1579 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT114571 NZ_CP033883:c1848-1579 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCAGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGTGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0