Detailed information of TA system TA114566

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33255..33508
• Replicon: plasmid p50579417_4
• Accession: NZ_CP033881
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EGM66_RS00960
• Protein ID: WP_001312851.1
• Coordinates: 33255..33404 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	33449..33508 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS00910	28608..29090	-	483	WP_001311056.1	hypothetical protein	-
EGM66_RS00915	29207..30055	-	849	WP_060578162.1	3'-5' exonuclease	-
EGM66_RS00920	30090..30371	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGM66_RS00925	30368..30637	-	270	WP_001481137.1	hypothetical protein	-
EGM66_RS00940	31553..32410	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
EGM66_RS00945	32403..32477	-	75	WP_001365705.1	RepA leader peptide Tap	-
EGM66_RS00955	32722..32970	-	249	WP_000083839.1	replication regulatory protein RepA	-
EGM66_RS00960	33255..33404	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33449..33508	+	60	NuclAT_1	-	Antitoxin
-	33449..33508	+	60	NuclAT_1	-	Antitoxin
-	33449..33508	+	60	NuclAT_1	-	Antitoxin
-	33449..33508	+	60	NuclAT_1	-	Antitoxin
EGM66_RS00970	33651..34124	-	474	WP_016240489.1	hypothetical protein	-
EGM66_RS00975	34279..34869	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
EGM66_RS00980	34907..35116	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
EGM66_RS00985	35162..35623	-	462	WP_001233838.1	thermonuclease family protein	-
EGM66_RS00990	35868..36080	-	213	WP_001309245.1	ANR family transcriptional regulator	-
EGM66_RS00995	36209..36769	-	561	WP_000139321.1	fertility inhibition protein FinO	-
EGM66_RS01000	36872..37732	-	861	WP_000704523.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61890	61890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T114566 WP_001312851.1 NZ_CP033881:c33404-33255 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T114566 NZ_CP033881:c33404-33255 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT114566 NZ_CP033881:33449-33508 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin