Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1171654..1172287 Replicon chromosome
Accession NZ_CP033875
Organism Caulobacter sp. FWC26

Toxin (Protein)


Gene name graT Uniprot ID A0A3Q9IG65
Locus tag CSW63_RS07085 Protein ID WP_062095669.1
Coordinates 1172000..1172287 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3S9VCW2
Locus tag CSW63_RS07080 Protein ID WP_062095671.1
Coordinates 1171654..1171968 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSW63_RS07050 1167145..1167624 + 480 WP_168193620.1 DNA starvation/stationary phase protection protein -
CSW63_RS07055 1167634..1168779 + 1146 Protein_1060 cell wall hydrolase -
CSW63_RS07060 1168895..1169902 - 1008 WP_062095680.1 thioredoxin-disulfide reductase -
CSW63_RS07065 1169963..1170151 + 189 WP_062095678.1 hypothetical protein -
CSW63_RS07070 1170177..1170428 + 252 WP_062095676.1 hypothetical protein -
CSW63_RS07075 1170501..1171553 + 1053 WP_062095673.1 pseudaminic acid synthase -
CSW63_RS07080 1171654..1171968 - 315 WP_062095671.1 HigA family addiction module antidote protein Antitoxin
CSW63_RS07085 1172000..1172287 - 288 WP_062095669.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CSW63_RS07090 1172362..1173222 - 861 WP_062095667.1 MBL fold metallo-hydrolase -
CSW63_RS07095 1173376..1174143 + 768 WP_062095665.1 sulfite exporter TauE/SafE family protein -
CSW63_RS07100 1174151..1175002 - 852 WP_062095663.1 SDR family NAD(P)-dependent oxidoreductase -
CSW63_RS07105 1175099..1176436 - 1338 WP_062095661.1 PAS domain S-box protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-74)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10594.16 Da        Isoelectric Point: 8.6582

>T114556 WP_062095669.1 NZ_CP033875:c1172287-1172000 [Caulobacter sp. FWC26]
MAIQTFKDLRAKAILEGVSSGKGFPSDLIRVAKRKLEMLEAAVVLNDLRTPPGNRLEALSGDRKGQHSIRVNDQFRLVFV
WTDAGPADVEFVDYH

Download         Length: 288 bp

>T114556 NZ_CP033875:c1172287-1172000 [Caulobacter sp. FWC26]
ATGGCGATCCAGACCTTCAAGGACCTGCGCGCGAAGGCCATCCTCGAAGGGGTGTCCTCCGGAAAAGGTTTTCCGTCCGA
CCTCATTAGGGTCGCCAAGCGCAAGTTGGAGATGCTGGAAGCCGCCGTCGTCCTGAACGACCTTCGCACGCCGCCGGGCA
ATCGGCTCGAAGCCTTGTCCGGAGACCGTAAGGGTCAGCATTCGATCCGGGTCAACGATCAGTTCCGGCTGGTCTTTGTC
TGGACCGACGCCGGACCGGCGGATGTCGAGTTCGTGGACTATCACTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11616.46 Da        Isoelectric Point: 7.2034

>AT114556 WP_062095671.1 NZ_CP033875:c1171968-1171654 [Caulobacter sp. FWC26]
MSKDIVQDNDPLAPVHPGEILLEEFLKPYGLTPGRVAARLHIARPRIEKLVRGQTPVTIDTALRLERLFGASAQFWLNLQ
NRYDLEMAKRGVAPDIASIEPFAA

Download         Length: 315 bp

>AT114556 NZ_CP033875:c1171968-1171654 [Caulobacter sp. FWC26]
ATGAGCAAGGATATCGTGCAGGACAACGATCCCCTCGCCCCGGTCCATCCGGGCGAAATCCTGCTGGAAGAGTTCCTGAA
GCCCTATGGCCTCACGCCCGGCCGCGTCGCGGCCCGGCTGCACATCGCTCGCCCTCGGATCGAGAAGCTTGTTCGGGGGC
AAACCCCGGTGACCATCGACACGGCTCTGCGCCTGGAGCGCCTGTTCGGCGCCAGCGCGCAGTTCTGGCTCAATCTGCAG
AACCGCTACGACTTGGAAATGGCCAAGCGAGGCGTCGCGCCGGACATCGCGAGCATCGAGCCGTTCGCGGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q9IG65

Antitoxin

Source ID Structure
AlphaFold DB A0A3S9VCW2

References