Detailed information of TA system TA114092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 60718..61252
• Replicon: plasmid unnamed
• Accession: NZ_CP033708
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_583

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: EGX39_RS00425
• Protein ID: WP_002213360.1
• Coordinates: 60953..61252 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: EGX39_RS00420
• Protein ID: WP_012414146.1
• Coordinates: 60718..60960 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX39_RS00400	57283..58449	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
EGX39_RS00405	58446..59411	+	966	WP_172957062.1	ParB/RepB/Spo0J family plasmid partition protein	-
EGX39_RS21455	59571..59852	+	282	WP_157868821.1	hypothetical protein	-
EGX39_RS21460	60002..60175	-	174	WP_002213357.1	hypothetical protein	-
EGX39_RS00415	60260..60552	+	293	Protein_78	IS6 family transposase	-
EGX39_RS00420	60718..60960	+	243	WP_012414146.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EGX39_RS00425	60953..61252	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX39_RS00430	61392..62051	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
EGX39_RS00435	62245..62637	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
EGX39_RS00440	62700..63344	-	645	Protein_83	IS3 family transposase	-
EGX39_RS00445	63447..64619	+	1173	WP_011191364.1	IS21 family transposase	-
EGX39_RS00450	64619..65017	+	399	Protein_85	ATP-binding protein	-
EGX39_RS00455	65395..65820	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yadA / yopO/ypkA / yopJ/yopP / yopH / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH	1..69970	69970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T114092 WP_002213360.1 NZ_CP033708:60953-61252 [Yersinia pseudotuberculosis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T114092 NZ_CP033708:60953-61252 [Yersinia pseudotuberculosis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8807.12 Da
• Isoelectric Point: 6.8783

>AT114092 WP_012414146.1 NZ_CP033708:60718-60960 [Yersinia pseudotuberculosis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKGTSV

Nucleotide

• Length: 243 bp

>AT114092 NZ_CP033708:60718-60960 [Yersinia pseudotuberculosis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGGTACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8