Detailed information of TA system TA113812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 111862..112519
• Replicon: plasmid pVCR94
• Accession: NZ_CP033514
• Organism: Vibrio cholerae strain E4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: EEL44_RS05990
• Protein ID: WP_000270043.1
• Coordinates: 112169..112519 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EEL44_RS05985
• Protein ID: WP_000124640.1
• Coordinates: 111862..112164 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL44_RS05935	107487..107915	+	429	WP_000591074.1	hypothetical protein	-
EEL44_RS05940	107972..108331	+	360	WP_000422768.1	hypothetical protein	-
EEL44_RS05945	108331..108777	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
EEL44_RS05950	108774..109292	+	519	WP_000210756.1	nitrite reductase	-
EEL44_RS05955	109292..109522	+	231	WP_000972663.1	hypothetical protein	-
EEL44_RS05960	109509..110366	+	858	WP_001167032.1	hypothetical protein	-
EEL44_RS05970	110597..111124	+	528	WP_001236377.1	thermonuclease family protein	-
EEL44_RS05975	111182..111454	+	273	WP_001043047.1	HU family DNA-binding protein	-
EEL44_RS05980	111542..111835	+	294	WP_001239997.1	chromosome segregation protein ParM	-
EEL44_RS05985	111862..112164	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EEL44_RS05990	112169..112519	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EEL44_RS05995	112682..113230	+	549	WP_001061574.1	transcriptional regulator	-
EEL44_RS06005	113571..113765	+	195	WP_000343597.1	hypothetical protein	-
EEL44_RS06010	113776..114147	+	372	WP_000516916.1	hypothetical protein	-
EEL44_RS06015	114140..114610	+	471	WP_001281821.1	hypothetical protein	-
EEL44_RS06020	114625..114960	-	336	WP_000683476.1	hypothetical protein	-
EEL44_RS06025	115057..115545	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
EEL44_RS06030	115548..116045	+	498	WP_000062185.1	hypothetical protein	-
EEL44_RS06035	116311..117015	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(C) / tet(B) / catA1 / aph(3'')-Ib / aph(6)-Id / blaCARB-4 / dfrA15 / qacE / sul1 / sul2	-	1..171829	171829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T113812 WP_000270043.1 NZ_CP033514:c112519-112169 [Vibrio cholerae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T113812 NZ_CP033514:c112519-112169 [Vibrio cholerae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT113812 WP_000124640.1 NZ_CP033514:c112164-111862 [Vibrio cholerae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT113812 NZ_CP033514:c112164-111862 [Vibrio cholerae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9