Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1358540..1359341 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag EEL47_RS07055 Protein ID WP_001094430.1
Coordinates 1358964..1359341 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag EEL47_RS07050 Protein ID WP_001285620.1
Coordinates 1358540..1358917 (+) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS07010 (1355161) 1355161..1355616 + 456 WP_005051844.1 IrmA family protein -
EEL47_RS26175 (1355759) 1355759..1355848 + 90 WP_112031555.1 DUF905 family protein -
EEL47_RS07025 (1356027) 1356027..1356845 + 819 WP_001177758.1 DUF932 domain-containing protein -
EEL47_RS07035 (1357187) 1357187..1357660 + 474 WP_000855059.1 antirestriction protein -
EEL47_RS07040 (1357676) 1357676..1358152 + 477 WP_001387238.1 RadC family protein -
EEL47_RS07045 (1358239) 1358239..1358460 + 222 WP_000692350.1 DUF987 domain-containing protein -
EEL47_RS07050 (1358540) 1358540..1358917 + 378 WP_001285620.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
EEL47_RS07055 (1358964) 1358964..1359341 + 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
EEL47_RS07060 (1359338) 1359338..1359826 + 489 WP_000761715.1 DUF5983 family protein -
EEL47_RS07065 (1359846) 1359846..1360043 + 198 WP_000772029.1 DUF957 domain-containing protein -
EEL47_RS07070 (1360128) 1360128..1360970 + 843 WP_001290187.1 DUF4942 domain-containing protein -
EEL47_RS07075 (1361747) 1361747..1361992 + 246 WP_000875412.1 hypothetical protein -
EEL47_RS07080 (1362395) 1362395..1363557 + 1163 WP_094081514.1 IS3-like element IS3 family transposase -
EEL47_RS07085 (1363661) 1363661..1364128 - 468 WP_011069513.1 prepilin peptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T113778 WP_001094430.1 NZ_CP033510:1358964-1359341 [Shigella flexneri]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T113778 NZ_CP033510:1358964-1359341 [Shigella flexneri]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT113778 WP_001285620.1 NZ_CP033510:1358540-1358917 [Shigella flexneri]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT113778 NZ_CP033510:1358540-1358917 [Shigella flexneri]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7


Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References