Detailed information of TA system TA113580

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4361265..4362100
• Replicon: chromosome
• Accession: NZ_CP033378
• Organism: Escherichia coli strain L73

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EB670_RS21495
• Protein ID: WP_057109034.1
• Coordinates: 4361723..4362100 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1E5MC43
• Locus tag: EB670_RS21490
• Protein ID: WP_063112944.1
• Coordinates: 4361265..4361633 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB670_RS21450	4356563..4357243	+	681	WP_001282919.1	WYL domain-containing protein	-
EB670_RS21455	4357391..4358068	+	678	WP_001097301.1	hypothetical protein	-
EB670_RS21460	4358074..4358307	+	234	WP_001278287.1	DUF905 family protein	-
EB670_RS21465	4358397..4359215	+	819	WP_122927395.1	DUF945 domain-containing protein	-
EB670_RS21470	4359297..4359776	+	480	WP_000860074.1	antirestriction protein	-
EB670_RS21475	4359792..4360268	+	477	WP_001366830.1	RadC family protein	-
EB670_RS21480	4360331..4360552	+	222	WP_000692323.1	DUF987 domain-containing protein	-
EB670_RS21485	4360571..4361215	+	645	WP_045146861.1	hypothetical protein	-
EB670_RS21490	4361265..4361633	+	369	WP_063112944.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EB670_RS21495	4361723..4362100	+	378	WP_057109034.1	TA system toxin CbtA family protein	Toxin
EB670_RS21500	4362097..4362276	+	180	Protein_4164	hypothetical protein	-
EB670_RS21505	4362322..4362519	+	198	WP_023563519.1	DUF957 domain-containing protein	-
EB670_RS21510	4362604..4363446	+	843	WP_122927396.1	DUF4942 domain-containing protein	-
EB670_RS21515	4364195..4365733	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4349840..4373545	23705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.86 Da
• Isoelectric Point: 6.8517

>T113580 WP_057109034.1 NZ_CP033378:4361723-4362100 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T113580 NZ_CP033378:4361723-4362100 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13724.52 Da
• Isoelectric Point: 6.8270

>AT113580 WP_063112944.1 NZ_CP033378:4361265-4361633 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT113580 NZ_CP033378:4361265-4361633 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E5MC43