Detailed information of TA system TA113502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4184759..4186442
• Replicon: chromosome
• Accession: NZ_CP033250
• Organism: Escherichia coli strain ECCHD184

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: I2UPR9
• Locus tag: D3W51_RS20415
• Protein ID: WP_000361917.1
• Coordinates: 4185525..4186442 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: D3W51_RS20410
• Protein ID: WP_024243514.1
• Coordinates: 4184759..4185532 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W51_RS20380	4180817..4181797	+	981	WP_000338799.1	sialate O-acetylesterase	-
D3W51_RS20385	4181805..4181927	-	123	WP_001338066.1	hypothetical protein	-
D3W51_RS20390	4182009..4182881	+	873	WP_122224781.1	HNH endonuclease	-
D3W51_RS20395	4182816..4183513	-	698	Protein_3959	IS1 family transposase	-
D3W51_RS20400	4183529..4184167	+	639	Protein_3960	phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein	-
D3W51_RS20405	4184343..4184671	-	329	Protein_3961	IS3 family transposase	-
D3W51_RS20410	4184759..4185532	+	774	WP_024243514.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
D3W51_RS20415	4185525..4186442	+	918	WP_000361917.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
D3W51_RS24850	4186736..4186903	+	168	WP_001356018.1	hypothetical protein	-
D3W51_RS20420	4186956..4187552	-	597	Protein_3965	hypothetical protein	-
D3W51_RS20425	4187623..4188333	-	711	WP_072841475.1	integrase arm-type DNA-binding domain-containing protein	-
D3W51_RS20435	4188814..4189833	+	1020	WP_001299662.1	NADPH-dependent aldehyde reductase Ahr	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4182816..4183193	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34963.04 Da
• Isoelectric Point: 4.7716

>T113502 WP_000361917.1 NZ_CP033250:4185525-4186442 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T113502 NZ_CP033250:4185525-4186442 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACTTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGGAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGGACACTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGGTGGCAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACACAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGTGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCACGTTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCACGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGATGTCATTTAAACGCGGCGAGCCAGACTGGAATTTAGCGCCAGAGCCCCA
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATGTCGGAGGAAAAGCGCGCGCAGTCACTGG
AGAAACTAGAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29319.73 Da
• Isoelectric Point: 9.9889

>AT113502 WP_024243514.1 NZ_CP033250:4184759-4185532 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPGVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT113502 NZ_CP033250:4184759-4185532 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGGCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Structures

Toxin

• AlphaFold DB: A0A0H0PS08