Detailed information of TA system TA113408

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2131370..2131566
• Replicon: chromosome
• Accession: NZ_CP033213
• Organism: Clostridioides difficile strain CD10010

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: EBB60_RS10395
• Protein ID: WP_003424190.1
• Coordinates: 2131414..2131566 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	2131370..2131470 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBB60_RS10365	2126525..2126719	-	195	WP_003424168.1	hypothetical protein	-
EBB60_RS10375	2127256..2127636	-	381	WP_003424180.1	hypothetical protein	-
EBB60_RS10380	2127629..2127886	-	258	WP_003424182.1	hypothetical protein	-
EBB60_RS10385	2127873..2128106	-	234	WP_003424184.1	hypothetical protein	-
EBB60_RS10390	2128294..2128482	+	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
-	2131370..2131470	+	101	NuclAT_0	-	Antitoxin
EBB60_RS10395	2131414..2131566	-	153	WP_003424190.1	hypothetical protein	Toxin
EBB60_RS10400	2132086..2132328	+	243	WP_003424191.1	hypothetical protein	-
EBB60_RS10405	2132394..2132939	+	546	WP_077699404.1	site-specific integrase	-
EBB60_RS10410	2133484..2134329	+	846	WP_003424194.1	DUF4878 domain-containing protein	-
EBB60_RS10415	2134553..2134717	-	165	WP_003424195.1	hypothetical protein	-
EBB60_RS10420	2135249..2135580	-	332	Protein_1914	carboxymuconolactone decarboxylase family protein	-
EBB60_RS10425	2135840..2136481	-	642	WP_003424198.1	SdpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2116317..2159736	43419

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T113408 WP_003424190.1 NZ_CP033213:c2131566-2131414 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T113408 NZ_CP033213:c2131566-2131414 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT113408 NZ_CP033213:2131370-2131470 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin