Detailed information of TA system TA113351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4728675..4729264
• Replicon: chromosome
• Accession: NZ_CP033188
• Organism: Xanthomonas oryzae pv. oryzae strain PXO513

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: EBA25_RS23515
• Protein ID: WP_012443754.1
• Coordinates: 4728983..4729264 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA25_RS23510
• Protein ID: WP_011260746.1
• Coordinates: 4728675..4728965 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA25_RS23470	4723963..4724436	-	474	Protein_4226	DUF4123 domain-containing protein	-
EBA25_RS23475	4724439..4724873	-	435	WP_011409747.1	PAAR domain-containing protein	-
EBA25_RS23480	4724989..4725234	-	246	WP_069960049.1	hypothetical protein	-
EBA25_RS23490	4725570..4725902	+	333	WP_011260743.1	hypothetical protein	-
EBA25_RS23500	4726352..4727746	-	1395	WP_027703652.1	nucleoside hydrolase	-
EBA25_RS23510	4728675..4728965	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
EBA25_RS23515	4728983..4729264	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA25_RS23520	4729359..4731611	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T113351 WP_012443754.1 NZ_CP033188:c4729264-4728983 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113351 NZ_CP033188:c4729264-4728983 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT113351 WP_011260746.1 NZ_CP033188:c4728965-4728675 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113351 NZ_CP033188:c4728965-4728675 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5