Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3095957..3096552 Replicon chromosome
Accession NZ_CP033158
Organism Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag EAF06_RS14775 Protein ID WP_000239579.1
Coordinates 3096202..3096552 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag EAF06_RS14770 Protein ID WP_001223208.1
Coordinates 3095957..3096208 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAF06_RS14760 3091622..3095401 + 3780 WP_000060978.1 autotransporter assembly complex protein TamB -
EAF06_RS14765 3095404..3095745 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EAF06_RS14770 3095957..3096208 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EAF06_RS14775 3096202..3096552 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
EAF06_RS14780 3096632..3097162 - 531 WP_000055072.1 inorganic diphosphatase -
EAF06_RS14785 3097472..3098428 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EAF06_RS14790 3098568..3100070 + 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
EAF06_RS14795 3100084..3101106 + 1023 WP_001295197.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T113270 WP_000239579.1 NZ_CP033158:3096202-3096552 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T113270 NZ_CP033158:3096202-3096552 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT113270 WP_001223208.1 NZ_CP033158:3095957-3096208 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT113270 NZ_CP033158:3095957-3096208 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References