Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1681343..1682175 Replicon chromosome
Accession NZ_CP033158
Organism Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)


Gene name yeeV Uniprot ID A7ZVJ9
Locus tag EAF06_RS08005 Protein ID WP_000854765.1
Coordinates 1681801..1682175 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag EAF06_RS08000 Protein ID WP_001295723.1
Coordinates 1681343..1681711 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAF06_RS07965 1677007..1677687 + 681 WP_001282921.1 WYL domain-containing protein -
EAF06_RS07970 1677835..1678512 + 678 WP_001097302.1 hypothetical protein -
EAF06_RS07975 1678518..1678751 + 234 WP_001278293.1 DUF905 family protein -
EAF06_RS07980 1678841..1679659 + 819 WP_001175165.1 DUF945 domain-containing protein -
EAF06_RS07985 1679925..1680404 + 480 WP_057109541.1 antirestriction protein -
EAF06_RS07990 1680420..1680896 + 477 WP_001186774.1 RadC family protein -
EAF06_RS07995 1680959..1681180 + 222 WP_000692341.1 DUF987 domain-containing protein -
EAF06_RS08000 1681343..1681711 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EAF06_RS08005 1681801..1682175 + 375 WP_000854765.1 TA system toxin CbtA family protein Toxin
EAF06_RS08010 1682175..1682378 + 204 WP_001398797.1 hypothetical protein -
EAF06_RS08015 1682403..1682597 + 195 WP_024181941.1 DUF957 domain-containing protein -
EAF06_RS08020 1682682..1683524 + 843 WP_022645115.1 DUF4942 domain-containing protein -
EAF06_RS08025 1683805..1684341 - 537 WP_000942785.1 GspM family type II secretion system protein YghD -
EAF06_RS08030 1684343..1685521 - 1179 WP_000094991.1 type II secretion system protein GspL -
EAF06_RS08035 1685518..1686495 - 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -
EAF06_RS08040 1686498..1687097 - 600 WP_001255034.1 type II secretion system minor pseudopilin GspJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1524833..1683651 158818


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 13970.98 Da        Isoelectric Point: 7.2909

>T113265 WP_000854765.1 NZ_CP033158:1681801-1682175 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T113265 NZ_CP033158:1681801-1682175 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT113265 WP_001295723.1 NZ_CP033158:1681343-1681711 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT113265 NZ_CP033158:1681343-1681711 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A7ZVJ9


Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References