Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 528452..528954 Replicon chromosome
Accession NZ_CP033158
Organism Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EAF06_RS02645 Protein ID WP_000767829.1
Coordinates 528452..528706 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EAF06_RS02650 Protein ID WP_001259255.1
Coordinates 528703..528954 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAF06_RS02615 523467..523694 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EAF06_RS02620 523708..524766 - 1059 WP_000492321.1 transport protein YeeE -
EAF06_RS02625 524945..526303 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EAF06_RS02630 526293..526355 - 63 WP_010723108.1 membrane protein YoeI -
EAF06_RS02635 526570..527499 - 930 WP_000803351.1 LysR family transcriptional regulator -
EAF06_RS02640 527545..528369 - 825 WP_000754749.1 SDR family oxidoreductase -
EAF06_RS02645 528452..528706 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EAF06_RS02650 528703..528954 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EAF06_RS02655 529238..529288 + 51 WP_001364200.1 his operon leader peptide -
EAF06_RS02660 529434..530333 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
EAF06_RS02665 530339..531643 + 1305 WP_001446927.1 histidinol dehydrogenase -
EAF06_RS02670 531640..532710 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
EAF06_RS02675 532710..533777 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T113262 WP_000767829.1 NZ_CP033158:c528706-528452 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T113262 NZ_CP033158:c528706-528452 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT113262 WP_001259255.1 NZ_CP033158:c528954-528703 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT113262 NZ_CP033158:c528954-528703 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References