Detailed information of TA system TA113224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 226938..227523
• Replicon: plasmid pOXA23_010062
• Accession: NZ_CP033130
• Organism: Acinetobacter wuhouensis strain WCHAW010062

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: CDG68_RS02115
• Protein ID: WP_000897307.1
• Coordinates: 227203..227523 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG68_RS02110
• Protein ID: WP_000369781.1
• Coordinates: 226938..227210 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG68_RS02090	222096..222941	-	846	WP_087554577.1	hypothetical protein	-
CDG68_RS02095	223229..223840	+	612	WP_015060246.1	recombinase family protein	-
CDG68_RS02100	224030..224914	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
CDG68_RS02105	224970..226445	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
CDG68_RS02110	226938..227210	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
CDG68_RS02115	227203..227523	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG68_RS02120	227663..228082	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
CDG68_RS02125	228340..228864	-	525	WP_039090898.1	plasmid replication DNA-binding protein	-
CDG68_RS02130	228943..229680	-	738	Protein_224	replication initiation protein RepM	-
CDG68_RS02135	229685..230263	+	579	Protein_225	IS3 family transposase	-
CDG68_RS02140	230273..231289	-	1017	WP_005006273.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / blaOXA-23 / blaOXA-58 / cmlB1 / mph(E) / msr(E) / aac(3)-IId	-	1..311749	311749
	-	inside	IScluster/Tn	blaOXA-58 / cmlB1 / mph(E) / msr(E) / aac(3)-IId	-	211356..252770	41414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T113224 WP_000897307.1 NZ_CP033130:c227523-227203 [Acinetobacter wuhouensis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T113224 NZ_CP033130:c227523-227203 [Acinetobacter wuhouensis]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT113224 WP_000369781.1 NZ_CP033130:c227210-226938 [Acinetobacter wuhouensis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT113224 NZ_CP033130:c227210-226938 [Acinetobacter wuhouensis]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3