Detailed information of TA system TA112944

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3972850..3973685
• Replicon: chromosome
• Accession: NZ_CP032992
• Organism: Escherichia coli strain W5-6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LDL9
• Locus tag: D9V54_RS19665
• Protein ID: WP_001094444.1
• Coordinates: 3972850..3973227 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: D9V54_RS19670
• Protein ID: WP_001285607.1
• Coordinates: 3973317..3973685 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V54_RS19645	3970225..3971490	-	1266	WP_000772679.1	integrase arm-type DNA-binding domain-containing protein	-
D9V54_RS25110	3971917..3972063	-	147	WP_001290178.1	hypothetical protein	-
D9V54_RS19655	3972148..3972345	-	198	WP_001327226.1	DUF957 domain-containing protein	-
D9V54_RS19660	3972365..3972853	-	489	WP_000761685.1	hypothetical protein	-
D9V54_RS19665	3972850..3973227	-	378	WP_001094444.1	TA system toxin CbtA family protein	Toxin
D9V54_RS19670	3973317..3973685	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D9V54_RS19675	3973765..3973986	-	222	WP_000692350.1	DUF987 domain-containing protein	-
D9V54_RS19680	3974073..3974549	-	477	WP_001384029.1	RadC family protein	-
D9V54_RS19685	3974564..3975049	-	486	WP_000213722.1	antirestriction protein	-
D9V54_RS19690	3975141..3975959	-	819	WP_001175175.1	DUF945 domain-containing protein	-
D9V54_RS19695	3976049..3976258	-	210	WP_032150870.1	DUF905 family protein	-
D9V54_RS19700	3976288..3976965	-	678	WP_001097301.1	hypothetical protein	-
D9V54_RS19705	3977113..3977793	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	3913363..3989314	75951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14087.02 Da
• Isoelectric Point: 7.3523

>T112944 WP_001094444.1 NZ_CP032992:c3973227-3972850 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T112944 NZ_CP032992:c3973227-3972850 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT112944 WP_001285607.1 NZ_CP032992:c3973685-3973317 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT112944 NZ_CP032992:c3973685-3973317 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: B1LDL9

Antitoxin

• AlphaFold DB: A0A1M0JLU8