Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3623897..3624515 Replicon chromosome
Accession NZ_CP032989
Organism Escherichia coli strain W2-5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D9V55_RS18075 Protein ID WP_001291435.1
Coordinates 3624297..3624515 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D9V55_RS18070 Protein ID WP_000344800.1
Coordinates 3623897..3624271 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V55_RS18060 3618986..3620179 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D9V55_RS18065 3620202..3623351 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
D9V55_RS18070 3623897..3624271 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D9V55_RS18075 3624297..3624515 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
D9V55_RS18080 3624686..3625237 + 552 WP_000102578.1 maltose O-acetyltransferase -
D9V55_RS18085 3625353..3625823 + 471 WP_000136192.1 YlaC family protein -
D9V55_RS18090 3625987..3626436 + 450 Protein_3419 cyclic-guanylate-specific phosphodiesterase PdeB -
D9V55_RS18095 3626476..3627702 - 1227 WP_001672025.1 transposase -
D9V55_RS18100 3627761..3628165 + 405 WP_001175008.1 IS200/IS605 family transposase -
D9V55_RS18105 3628243..3629355 + 1113 Protein_3422 cyclic-guanylate-specific phosphodiesterase PdeB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T112904 WP_001291435.1 NZ_CP032989:3624297-3624515 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T112904 NZ_CP032989:3624297-3624515 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT112904 WP_000344800.1 NZ_CP032989:3623897-3624271 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT112904 NZ_CP032989:3623897-3624271 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References