Detailed information of TA system TA112843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1730142..1730758
• Replicon: chromosome
• Accession: NZ_CP032916
• Organism: Enterobacter roggenkampii strain ECY546

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D8675_RS09440
• Protein ID: WP_084832108.1
• Coordinates: 1730387..1730758 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: D8675_RS09435
• Protein ID: WP_015569912.1
• Coordinates: 1730142..1730384 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8675_RS09405	1725561..1726031	+	471	WP_045372003.1	hypothetical protein	-
D8675_RS09410	1726120..1726719	+	600	WP_008501839.1	glucose-1-phosphatase	-
D8675_RS09415	1726713..1727594	+	882	WP_021242790.1	virulence factor BrkB family protein	-
D8675_RS09420	1727591..1728028	+	438	WP_025912308.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D8675_RS09425	1728073..1729014	+	942	WP_008501835.1	fatty acid biosynthesis protein FabY	-
D8675_RS09430	1729023..1729943	-	921	WP_129694063.1	alpha/beta hydrolase	-
D8675_RS09435	1730142..1730384	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
D8675_RS09440	1730387..1730758	+	372	WP_084832108.1	type II toxin-antitoxin system VapC family toxin	Toxin
D8675_RS09445	1730798..1731727	-	930	WP_129694064.1	formate dehydrogenase accessory protein FdhE	-
D8675_RS09450	1731724..1732359	-	636	WP_045372006.1	formate dehydrogenase cytochrome b556 subunit	-
D8675_RS09455	1732356..1733258	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13686.79 Da
• Isoelectric Point: 6.3290

>T112843 WP_084832108.1 NZ_CP032916:1730387-1730758 [Enterobacter roggenkampii]
MEHMAVFDTNILIDLFNNRVEAADAIENTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLVSRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T112843 NZ_CP032916:1730387-1730758 [Enterobacter roggenkampii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GAATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTGATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATCCTAGCTACAGCGCAGAGCAGAAAATGTCCGTTGGTTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT112843 WP_015569912.1 NZ_CP032916:1730142-1730384 [Enterobacter roggenkampii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT112843 NZ_CP032916:1730142-1730384 [Enterobacter roggenkampii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGCGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3