Detailed information of TA system TA112519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2638683..2639154
• Replicon: chromosome
• Accession: NZ_CP032808
• Organism: Escherichia coli strain ERL04-3476

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: D8Z68_RS13660
• Protein ID: WP_001303511.1
• Coordinates: 2638683..2638961 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: D8Z68_RS13665
• Protein ID: WP_001302048.1
• Coordinates: 2638963..2639154 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z68_RS13620	2633689..2633873	-	185	Protein_2603	DUF3596 domain-containing protein	-
D8Z68_RS13625	2633908..2634159	-	252	WP_000005552.1	excisionase family protein	-
D8Z68_RS13630	2634232..2636703	-	2472	WP_000048458.1	exonuclease	-
D8Z68_RS13635	2636796..2636987	-	192	WP_001090200.1	DUF1482 family protein	-
D8Z68_RS13640	2636984..2637172	-	189	WP_000449192.1	cell division inhibition protein DicB	-
D8Z68_RS13650	2637741..2637959	-	219	WP_001171930.1	hypothetical protein	-
D8Z68_RS13655	2638031..2638330	-	300	WP_001240334.1	hypothetical protein	-
D8Z68_RS13660	2638683..2638961	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8Z68_RS13665	2638963..2639154	-	192	WP_001302048.1	hypothetical protein	Antitoxin
D8Z68_RS13670	2639175..2639546	-	372	WP_001169686.1	hypothetical protein	-
D8Z68_RS13675	2639644..2639946	+	303	WP_000172738.1	transcriptional regulator	-
D8Z68_RS13680	2639943..2640368	+	426	WP_000693943.1	Rha family transcriptional regulator	-
D8Z68_RS13685	2640391..2641353	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
D8Z68_RS13690	2641360..2642100	+	741	WP_000788938.1	ATP-binding protein	-
D8Z68_RS13695	2642126..2642895	+	770	Protein_2617	DUF1627 domain-containing protein	-
D8Z68_RS13700	2642911..2643306	+	396	WP_001118159.1	DUF977 family protein	-
D8Z68_RS13705	2643363..2643947	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espX7/nleL / espN / espK	2631522..2685912	54390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T112519 WP_001303511.1 NZ_CP032808:c2638961-2638683 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T112519 NZ_CP032808:c2638961-2638683 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT112519 WP_001302048.1 NZ_CP032808:c2639154-2638963 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT112519 NZ_CP032808:c2639154-2638963 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0