Detailed information of TA system TA112001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3260658..3261259
• Replicon: chromosome
• Accession: NZ_CP032617
• Organism: Bradyrhizobium diazoefficiens strain 110spc4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: Bdiaspc4_RS15535
• Protein ID: WP_011085796.1
• Coordinates: 3260978..3261259 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: Bdiaspc4_RS15530
• Protein ID: WP_011085795.1
• Coordinates: 3260658..3260966 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bdiaspc4_RS15515	3255904..3258492	+	2589	WP_011085792.1	MMPL family transporter	-
Bdiaspc4_RS15520	3258460..3259116	-	657	WP_038965759.1	DUF2147 domain-containing protein	-
Bdiaspc4_RS15525	3259258..3260649	+	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
Bdiaspc4_RS15530	3260658..3260966	-	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
Bdiaspc4_RS15535	3260978..3261259	-	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Bdiaspc4_RS15540	3261331..3262098	-	768	WP_011085797.1	anti-sigma factor	-
Bdiaspc4_RS15545	3262095..3262643	-	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
Bdiaspc4_RS15550	3262771..3263106	-	336	WP_011085799.1	cupredoxin family copper-binding protein	-
Bdiaspc4_RS15555	3263132..3264076	-	945	WP_011085800.1	metallophosphoesterase	-
Bdiaspc4_RS15560	3264256..3265122	+	867	WP_011085801.1	helix-turn-helix transcriptional regulator	-
Bdiaspc4_RS15565	3265145..3265468	-	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
Bdiaspc4_RS15570	3265514..3265876	-	363	WP_011085803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T112001 WP_011085796.1 NZ_CP032617:c3261259-3260978 [Bradyrhizobium diazoefficiens]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T112001 NZ_CP032617:c3261259-3260978 [Bradyrhizobium diazoefficiens]
GTGATCAGAACGTTTCGGGACAAAACGACTGAGGCAGTCTTCGACGGAGAAAGTCCCAAAGGCTTTCCAGCCGATCTCGT
GAAGGTTGCACGCCGGAAACTGCGTTATCTGCACGCTGCCGGCGAGCTCGGCGATCTAAGGGCGCCGCCCGGCAACCGTC
TGGAGGCCCTGAGCGGCAATCGCAAGGGACAGCACTCGATCAGGATCAATGATCAGTTCCGAGTGTGCTTCATCTGGACA
CCGCAAGGGCCCGTCGAGGTCGAGATTGTGGACTATCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT112001 WP_011085795.1 NZ_CP032617:c3260966-3260658 [Bradyrhizobium diazoefficiens]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT112001 NZ_CP032617:c3260966-3260658 [Bradyrhizobium diazoefficiens]
ATGGCCAAAAAACTCAAGCCCATGCACCCCGGTGAGGTTCTACGGGAGGAGTTTCTAGTCCCACTGAACATGTCCGCTGG
CGCGCTCGCCAAGACGTGCGGCTTGCCGCGCACGCGCATCGAGCGGATCGCGAATGAGCAGACCGGAATCACCGCCGACA
CCGCGCTTCGACTGGCCAAGGCGCTCGGCACAACGGCCGAACTCTGGCTCAATCTTCAGAATGATTATGACATCCAGATC
GCGAAGCGCGATCTCGGCAAGGCACTTGACCGCATCGAGACCGTGAACAGGCCGCAAGCGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2